CHANGES IN CHLOROPHYLL CONTENT AND ORGANIZATION DURING SENESCENCE OF THE PRIMARY LEAVES OF PHASEOLUS-VULGARIS L IN RELATION TO PHOTOSYNTHETIC ELECTRON-TRANSPORT
CHANGES IN CHLOROPHYLL CONTENT AND ORGANIZATION DURING SENESCENCE OF THE PRIMARY LEAVES OF PHASEOLUS-VULGARIS L IN RELATION TO PHOTOSYNTHETIC ELECTRON-TRANSPORT
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DOI：
10.1093/jxb/32.5.1009
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发表时间：
1981-01-01
影响因子：
6.9
通讯作者：
WOOLHOUSE, HW
中科院分区：
文献类型：
--
作者：
JENKINS, GI;BAKER, NR;WOOLHOUSE, HW
在衰老过程中，胡杨初生叶叶绿素含量大幅度下降。从成熟和衰老的叶片中分离出的叶绿体得到了非常相似的光饱和曲线的电子传递反应，涉及PS [光系统] I或PS II，这表明与每个反应中心的叶绿素分子的平均数并没有改变衰老过程中。显然，反应中心停止功能的同时，或可能之前，他们的天线叶绿素从类囊体膜上丢失，和百分比减少功能反应中心的数量每叶至少是一样大的百分比减少叶片叶绿素含量。用十二烷基硫酸钠处理样品，通过电泳检测类囊体膜制备物的叶绿素蛋白组成。在较老的叶片中，较小比例的叶绿素应用于聚丙烯酰胺凝胶与P700-叶绿素a-蛋白复合物。在77 ° C下，在734 nm处的发射也有下降。完整叶片组织衰老过程中的钾荧光光谱。老叶含有较小比例的叶绿素与PS I，这是一致的，观察到的叶片叶绿素a/B比在衰老过程中减少。叶绿素含量的变化对叶绿体进行光合电子传递的能力的影响进行了讨论。
A large decrease was observed in the chlorophyll content of the primary leaves of P. vulgaris during senescence. Chloroplasts isolated from mature and senescent leaves gave very similar light saturation curves for electron transport reactions involving either PS [photosystem] I or PS II, indicating that the average number of chlorophyll molecules associated with each reaction center did not change during senescence. Apparently, the reaction centers ceased to function at the same time as, or perhaps before, their antenna chlorophylls were lost from the thylakoid membrane, and the percentage decrease in the number of functional reaction centers per leaf was at least as great as the percentage decrease in the leaf chlorophyll content. The chlorophyll-protein composition of thylakoid membrane preparations was examined by electrophoresis of samples treated with sodium dodecyl sulfate. In older leaves a smaller proportion of the chlorophyll applied to polyacrylamide gels was associated with the P700-chlorophyll a-protein complex. There was also a decline in emission at 734 nm in the 77.degree. K fluorescence spectrum of intact leaf tissue during senescence. Older leaves contained a smaller proportion of chlorophylls associated with PS I, and this is consistent with the decrease observed in the leaf chlorophyll a/b ratio during senescence. The effect of these changes in chlorophyll content on the capacity of the chloroplast to carry out photosynthetic electron transport is discussed.