Epstein-Barr virus clonality in lymphomas occurring in patients with rheumatoid arthritis.
Epstein-Barr virus clonality in lymphomas occurring in patients with rheumatoid arthritis.
复制标题
类风湿性关节炎患者淋巴瘤中的 Epstein-Barr 病毒克隆。
DOI：
10.1002/art.1780390415
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发表时间：
1996
影响因子：
--
通讯作者：
Kamel,OW
中科院分区：
文献类型：
--
作者：
vandeRijn,M;Cleary,ML;Variakojis,D;Warnke,RA;Chang,PP;Kamel,OW
Objective.已提出爱泼斯坦-巴尔病毒（EBV）在类风湿性关节炎（RA）患者淋巴瘤发生中的致病作用。我们研究了2例RA患者的EBV阳性弥漫性大细胞淋巴瘤的分子特征。进行了免疫球蛋白基因重排的Southern印迹分析、EB病毒基因组克隆性的末端重复分析以及通过原位杂交和免疫过氧化物酶染色对淋巴瘤细胞进行双标记。结果。在这两种情况下，双标记研究将EBV基因组定位于恶性B细胞。两种肿瘤均含有克隆性免疫球蛋白基因重排和克隆性EBV基因组。我们的数据表明，EBV感染是这些肿瘤发展的早期步骤。这一发现进一步扩展了对这一淋巴瘤亚群与移植后淋巴瘤相似性的认识，并强调了免疫抑制在其发生中的作用。
Objective. A causative role for Epstein‐Barr virus (EBV) in the development of lymphoma in patients with rheumatoid arthritis (RA) has been proposed. We investigated the molecular features of EBV‐positive diffuse large cell lymphomas in 2 patients with RA.Methods. Southern blot analysis for immunoglobulin gene rearrangements, terminal repeat analysis for clonality of the EBV genome, and double‐labeling of the lymphoma cells by in situ hybridization and immuno‐peroxidase staining were performed.Results. In both cases, double‐labeling studies localized the EBV genome to the malignant B cells. Both neoplasms contained clonal immunoglobulin gene rearrangements and clonal EBV genomes.Conclusion. Our data indicate that EBV infection was an early step in the development of these neoplasms. The findings further extend knowledge on the similarity of this subset of lymphomas to posttransplantation lymphomas and emphasize the role of immunosuppression in their genesis.