Heterogeneous Diversity of Spacers within CRISPR (Clustered Regularly Interspaced Short Palindromic Repeats)
Heterogeneous Diversity of Spacers within CRISPR (Clustered Regularly Interspaced Short Palindromic Repeats)
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DOI：
10.1103/physrevlett.105.128102
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发表时间：
2010-09-14
影响因子：
8.6
通讯作者：
Deem, Michael W.
中科院分区：
文献类型：
--
作者：
He, Jiankui;Deem, Michael W.
在细菌和古细菌DNA中聚集规律间隔短回文重复序列（CRISPR）最近被证明是这些生物中一种新型的抗病毒免疫系统。我们在这里研究了选择压力下CRISPR中间隔序列的多样性。我们提出了一个种群动态模型来解释CRISPR的先导-近端更多样化和先导-远端更保守的生物学观察。这一结果与最近的实验结果一致。我们的研究结果表明，CRISPR间隔结构受到细菌面临的病毒挑战的影响，并提供了一个记录。
Clustered regularly interspaced short palindromic repeats (CRISPR) in bacterial and archaeal DNA have recently been shown to be a new type of antiviral immune system in these organisms. We here study the diversity of spacers in CRISPR under selective pressure. We propose a population dynamics model that explains the biological observation that the leader-proximal end of CRISPR is more diversified and the leader-distal end of CRISPR is more conserved. This result is shown to be in agreement with recent experiments. Our results show that the CRISPR spacer structure is influenced by and provides a record of the viral challenges that bacteria face.