CHARACTERIZATION OF STREPTOCOCCUS-GORDONII (STREPTOCOCCUS-SANGUIS) PK488 ADHESIN-MEDIATED COAGGREGATION WITH ACTINOMYCES-NAESLUNDII PK606
CHARACTERIZATION OF STREPTOCOCCUS-GORDONII (STREPTOCOCCUS-SANGUIS) PK488 ADHESIN-MEDIATED COAGGREGATION WITH ACTINOMYCES-NAESLUNDII PK606
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DOI：
10.1128/iai.58.9.3064-3072.1990
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发表时间：
1990-09-01
影响因子：
3.1
通讯作者：
ANDERSEN, RN
中科院分区：
文献类型：
--
作者：
KOLENBRANDER, PE;ANDERSEN, RN
格氏链球菌属间共聚集。利用内氏放线菌（Actinomycesnaeslundii）PK 488和内氏放线菌（Actinomycesnaeslundii）PK 606的Cog-突变株，研究了它们的共聚集特性。用抗S.戈登氏血清与链球菌Cog-细胞吸附。吸收的免疫球蛋白G特异性地阻断了链球菌-放线菌对的共聚集，但不影响链球菌与其他oaggregation伙伴的共聚集。在轻度超声处理的细胞悬浮液的上清液中发现了38千道尔顿的蛋白质，并用巴比妥钠或3-[（3-胆酰胺丙基）-二甲基铵基]-1-丙磺酸盐（CHAPS）从全细胞中提取。在其他几种口腔链球菌的超声处理细胞上清液中也发现了同样大小的免疫反应蛋白，这些链球菌也与A。Naeslundii PK606.抑制S.戈登氏菌PK 488-A.用测试的16种不同糖中的任何一种都没有观察到内氏PK 606共聚集。我们提出一个功能相似的粘附素介导的coaggregation与A。内氏菌PK 606由链球菌属的几个物种表达。
Intergeneric coaggregation of Streptococcus gordonii (S. sanguis) PK488 and Actinomyces naeslundii PK606 was studied by using coaggregation-defective (Cog-) mutants of both strains. A streptococcal protein of 38 kilodaltons was identified with anti-S. gordonii serum absorbed with Cog- cells of the streptococcus. Absorbed immunoglobulin G specifically blocked coaggregation of the streptococcus-actinomyces pair but did not affect the coaggregation of the streptococcus with other oaggregation partners. The 38-kilodalton protein was found in the supernatant of mild sonicated cell suspensions and was extracted from whole cells with sodium barbital or with 3-[(3-cholamidopropyl)-dimethylammonio]-1-propanesulfonate (CHAPS). An immunoreactive protein of the same size was found in sonicated cell supernatants of several other oral streptococci that also coaggregated with A. naeslundii PK606. Inhibition of S. gordonii PK488-A. naeslundii PK606 coaggregation was not observed with any of 16 different sugars tested. We propose that a functionally similar adhesin that mediates coaggregations with A. naeslundii PK606 is expressed by several species of the genus Streptococcus.