Murine gammaherpesvirus 68 contains two functional lytic origins of replication
Murine gammaherpesvirus 68 contains two functional lytic origins of replication
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DOI：
10.1128/jvi.02406-06
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发表时间：
2007-07-01
影响因子：
5.4
通讯作者：
Haas, Juergen
中科院分区：
文献类型：
--
作者：
Adler, Heiko;Steer, Beatrix;Haas, Juergen
通过质粒复制试验鉴定了来自基因组右侧的1.25 kbp DNA片段，该片段含有鼠γ疱疹病毒68（MHV-68）的裂解复制起点（oriLyt）。在这里，我们表明，通过使用MHV-68细菌人工染色体产生的缺失该oriLyt的重要部分的突变体MHV-68仅轻微减毒，并且仍然能够复制，但是在基因组左侧含有额外缺失的突变体是复制缺陷的。新鉴定的区域足以支持质粒复制，从而为第二个oriLyt提供了证据。
A 1.25-kbp DNA fragment from the right side of the genome containing the lytic origin of replication (oriLyt) of murine gammaherpesvirus 68 (MHV-68) has been identified by a plasmid replication assay. Here we show that a mutant MHV-68 with a deletion of an essential part of this oriLyt, generated by using an MHV-68 bacterial artificial chromosome, was only slightly attenuated and still able to replicate but that a mutant containing an additional deletion on the left side of the genome was replication deficient. The newly identified region was sufficient to support plasmid replication, thus providing evidence for a second oriLyt.