LIMITATIONS OF TETRAZOLIUM SALTS IN DELINEATING INFARCTED BRAIN
LIMITATIONS OF TETRAZOLIUM SALTS IN DELINEATING INFARCTED BRAIN
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DOI：
10.1007/bf00690469
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发表时间：
1984-01-01
影响因子：
12.7
通讯作者：
ZERVAS, NT
中科院分区：
文献类型：
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作者：
LISZCZAK, TM;HEDLEYWHYTE, ET;ZERVAS, NT
四唑盐是线粒体呼吸酶的组织化学指示剂，已被一些病理学家用于检测心肌梗死。探讨了该技术在检测实验性脑梗塞中的应用。用单侧颞叶和永久性脑血管闭塞法在猫、沙鼠和大鼠中制备脑缺血模型。在不同的时间段后，将动物处死，并将它们的脑与氯化2，3，5-三苯基，2 H-四唑（TTC）反应。光镜和电镜观察实验侧和对侧大脑半球。TTC染色的组织与组织学相关。在某些情况下，组织的组织学状况与TTC染色结果良好相关。由暂时闭塞的血管供应并通过光学显微镜和EM判断为梗死的脑区未染色。在这些区域中，< 6%的线粒体是完整的。在永久性血管闭塞（无复流）动物的脑组织中，尽管其他细胞器（如细胞核）被破坏，但线粒体是完整的。TTC染色这种线粒体，结果不能区分完全缺血情况下的脑（无复流）。这种染色方法的另一个缺点是梗塞后36小时，具有完整线粒体的巨噬细胞将取代受损的神经元并被染色。在理想的条件下，虽然这项技术可以检测到不可逆的脑损伤早在动脉闭塞后2.5小时。
Tetrazolium salts, histochemical indicators of mitochondrial respiratory enzymes, have been used by some pathologists to detect infarcts in myocardium. The utility of this technique in detecting experimental brain infarcts was explored. Infarcts were produced in cats, gerbils and rats by unilateral temporal and permanent cerebral vessel occlusion. After various time periods the animals were killed, and their brains were reacted with 2, 3, 5, triphenyl, 2H-tetrazolium chloride (TTC). The experimental and contralateral hemispheres were examined by light microscopy and EM. The TTC-stained tissue was correlated with histology. In some situations the histological condition of the tissue correlated well with the TTC staining results. Brain regions supplied by temporarily occluded vessels and judged infarcted by light microscopy and EM did not stain. In these regions < 6% of the mitochondria were intact. In brain tissue from animals with permanent vessel occlusion (no reflow) mitochondria were intact despite the fact that other cellular organelles, such as nuclei, were destroyed. TTC stained such mitochondria and as a result could not distinguish brain in complete ischemia situations (no reflow). Another drawback to this staining procedure was 36 h after infarction macrophages with intact mitochondria would replace damaged neurons and be stained. Under ideal conditions though this technique can detect irreversibly damaged brain as early as 2.5 h after artery occlusion.