1H NMR investigation of normal and osteoarthritic synovial fluid in the horse
1H NMR investigation of normal and osteoarthritic synovial fluid in the horse
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DOI：
10.3415/vcot-06-12-0101
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发表时间：
2008-01-01
影响因子：
1.3
通讯作者：
Crovace, A.
中科院分区：
文献类型：
--
作者：
Lacitignola, L.;Fanizzi, F. P.;Crovace, A.
质子磁共振波谱（H-1 NMR）已成功地用于许多生物流体的研究。本文提供的数据报告了正常马滑液与骨关节炎（OA）液的代谢特征。从22匹马的前肢球节关节采集了25份OA滑液样本和8份正常滑液样本，年龄在5至24岁之间。1H-1 NMR光谱用配备有在11特斯拉下工作的低温磁体的Bruker Avance DRX 500进行，并且“Mestre-C 4.9.9.6”软件用于分析光谱。该研究评估了OA滑液中乳酸盐、丙氨酸、乙酸盐、N-乙酰葡糖胺、丙酮酸盐、柠檬酸盐、肌酸/肌酐、甘油、HDL胆碱和α-葡萄糖的增加。与对照组相比，在患有OA的马的样本中观察到的变化以及在其他研究中发现的类似数据证实，该技术可能适用于关节代谢研究。它的实际应用可能是在评估治疗OA的运动马。
Proton magnetic resonance spectroscopy (H-1 NMR) has been successfully used in the study of many biological fluids. The data presented here report on the metabolic profiles of normal equine synovial fluids compared with osteoarthritic (OA) fluids. Twenty-five OA synovial fluid samples and eight normal ones were collected from the forelimb fetlock joint in 22 horses, aged between five and 24 years. H-1 NMR spectroscopy was carried out with a Bruker Avance DRX 500 equiped with a cryomagnet working at 11 Tesla, and 'Mestre-C 4.9.9.6' software was used to analyze the spectra. The study assessed the increase of lactate, alanine, acetate, N-acetylglucosamine, pyruvate, citrate, creatine/creatinine, glycerol, HDL choline, and alpha-glucose in OA synovial fluid. The variations observed in samples from horses with OA compared to those in the control group, and similar data found in other studies, confirm that this technique may be useful in the study of joint metabolism. Its practical application may be in the evaluation of the treatment of OA in athletic horses.