Combined Effects of 872 MHz Radiofrequency Radiation and Ferrous Chloride on Reactive Oxygen Species Production and DNA Damage in Human SH-SY5Y Neuroblastoma Cells
Combined Effects of 872 MHz Radiofrequency Radiation and Ferrous Chloride on Reactive Oxygen Species Production and DNA Damage in Human SH-SY5Y Neuroblastoma Cells
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DOI：
10.1002/bem.20580
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发表时间：
2010-09-01
影响因子：
1.9
通讯作者：
Naarala, Jonne
中科院分区：
文献类型：
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作者：
Luukkonen, Jukka;Juutilainen, Jukka;Naarala, Jonne
本研究的目的是探讨射频（RF）辐射和氯化亚铁（FeCl 2）对活性氧（ROS）的产生和DNA损伤的可能的协同效应。为了检测细胞内ROS的产生是否是DNA损伤的潜在机制，我们应用荧光探针DCFH-DA，通过碱性彗星试验定量DNA的完整性。使用连续波（CW）或类似于全球移动的通信系统（GSM）电话中使用的调制信号，以5 W/kg的比吸收率（SAR）进行872 MHz RF辐射暴露。包括四组：（1）假暴露（对照），（2）RF辐射，（3）化学处理，（4）化学处理和RF辐射。在ROS产生实验中，将人神经母细胞瘤（SH-SY 5 Y）细胞暴露于RF辐射和10 μ g/ml FeCl 2 1小时。在彗星试验实验中，暴露时间为3小时，并使用额外的化学品（0.015%马来酸二乙酯），使DNA损伤水平可观察到。化学处理导致统计学上显著的反应，但没有观察到来自CW或调制RF辐射对ROS产生、DNA损伤或细胞活力的影响。Bioelectromagnetics 31：417-424，2010. (C)2010 Wiley-Liss，Inc.
The aim of the present study was to investigate possible cooperative effects of radiofrequency (RF) radiation and ferrous chloride (FeCl2) on reactive oxygen species (ROS) production and DNA damage. In order to test intracellular ROS production as a possible underlying mechanism of DNA damage, we applied the fluorescent probe DCFH-DA. Integrity of DNA was quantified by alkaline comet assay. The exposures to 872 MHz RF radiation were conducted at a specific absorption rate (SAR) of 5 W/kg using continuous waves (CW) or a modulated signal similar to that used in Global System for Mobile Communications (GSM) phones. Four groups were included: (1) Sham exposure (control), (2) RF radiation, (3) Chemical treatment, (4) Chemical treatment, and RF radiation. In the ROS production experiments, human neuroblastoma (SH-SY5Y) cells were exposed to RF radiation and 10 mu g/ml FeCl2 for 1 h. In the comet assay experiments, the exposure time was 3 h and an additional chemical (0.015% diethyl maleate) was used to make DNA damage level observable. The chemical treatments resulted in statistically significant responses, but no effects from either CW or modulated RF radiation were observed on ROS production, DNA damage or cell viability. Bioelectromagnetics 31:417-424, 2010. (C) 2010 Wiley-Liss, Inc.