Laccase mediated biodegradation of 2,4-dichlorophenol using response surface methodology
Laccase mediated biodegradation of 2,4-dichlorophenol using response surface methodology
复制标题
DOI：
10.1016/j.chemosphere.2008.05.005
复制
发表时间：
2008-08-01
期刊：
影响因子：
8.8
通讯作者：
Banerjee, R.
中科院分区：
文献类型：
--
作者：
Bhattacharya, S. S.;Banerjee, R.
The effects of different environmental parameters, i.e., pH, temperature, time and enzyme concentration on the biodegradation of 2,4-dichlorophenol (2,4-DCP) in aqueous phase was evaluated with laccase from Pleurotus sp. using response surface methodology (RSM) in the present investigation. The Box-Behnken design of experiments was used to construct second order response surfaces with the investigated parameters. It was observed that the maximum degradation efficiency of similar to 98% was achieved at pH 6, temperature of 40 degrees C, time 9 h and an enzyme concentration of 8 IU ml(-1). The adequacy of the model was confirmed by the coefficient of multiple regression, R-2 and adjusted R-2 which were adjudged to be 87.9% and 73.6%, respectively indicating a reasonably good model for practical implementation. Despite the fact that many successful attempts have been taken in the past for biodegradation of 2,4-DCP using whole cells, the present study emphasizes the fastest biodegradation of 2,4-DCP, a potent xenobiotic compound. (c) 2008 Elsevier Ltd. All rights reserved.
