PRODUCTION OF 4 IDENTICAL CALVES BY THE SEPARATION OF BLASTOMERES FROM AN IN-VITRO DERIVED 4-CELL EMBRYO
PRODUCTION OF 4 IDENTICAL CALVES BY THE SEPARATION OF BLASTOMERES FROM AN IN-VITRO DERIVED 4-CELL EMBRYO
复制标题
DOI：
10.1136/vr.137.1.15
复制
发表时间：
1995-07-01
影响因子：
2.2
通讯作者：
BETTERIDGE, KJ
中科院分区：
文献类型：
--
作者：
JOHNSON, WH;LOSKUTOFF, NM;BETTERIDGE, KJ
将两个体外衍生的四细胞胚胎的卵裂球分离并分别转移到替代透明带中，然后与牛输卵管上皮细胞共培养五天直至胚芽形成。将成对的四分之一囊胚与滋养层囊泡共同移植到四头同步荷斯坦小母牛中，其中三头在妊娠 28 天时被诊断出怀孕，怀有双胞胎胎儿。四头基因相同的小牛在足月妊娠时通过选择性剖腹产分娩。一名妊娠在第 56 天时终止。
The blastomeres of two in vitro derived four-cell embryos were separated and transferred individually into surrogate zonae pellucidae, then co-cultured with bovine oviductal epithelial cells for five days until blastulation. Pairs of the quarter blastocysts were co-transferred with trophoblastic vesicles into each of four synchronised Holstein heifers, three of which were diagnosed pregnant at 28 days gestation, carrying twin fetuses. Four genetically identical bull calves were delivered by elective caesarean section at term pregnancy. One pregnancy was terminated at 56 days.