Oxidative stress as a potential biomarker for determining disease activity in patients with Rheumatoid Arthritis
Oxidative stress as a potential biomarker for determining disease activity in patients with Rheumatoid Arthritis
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DOI：
10.3109/10715762.2012.727991
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发表时间：
2012-12-01
影响因子：
3.3
通讯作者：
Chatterjee, Mitali
中科院分区：
文献类型：
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作者：
Kundu, Sunanda;Ghosh, Parashar;Chatterjee, Mitali
类风湿性关节炎是一种炎症性自身免疫性疾病，其中氧化应激被认为是导致关节组织损伤的原因。为了确定血液中氧化还原状态的测量是否反映了类风湿关节炎患者炎症部位的氧化状态，我们同时用分光光度法和/或流式细胞术检测了他们的氧化状态。外周血和滑膜浸润中性粒细胞中总活性氧（ROS）、超氧化物和羟基自由基的基础水平均显著升高，且呈强正相关；然而，两种来源的单核细胞中产生的活性氮种类RNS并没有明显增加。此外，滑膜浸润中性粒细胞超氧化物水平升高与滑膜液NADPH氧化酶活性呈正相关。此外，外周血和滑膜浸润中产生的ROS与DAS 28和CRP/anti-CCP水平呈正相关，其测量可作为RA患者炎症程度的间接测量。
Rheumatoid arthritis is an inflammatory, autoimmune disease where oxidative stress has been proposed to contribute to the joint tissue damage. To establish whether measurement of the redox status in blood mirrors the oxidant status at sites of infl ammation in patients with rheumatoid arthritis, we concomitantly examined their oxidant status by spectrophotometry and/or flow cytometry. The basal levels of total reactive oxygen species (ROS), superoxide and hydroxyl radicals were significantly raised in neutrophils sourced from peripheral blood and synovial infiltrate, as also showed a strong positive correlation; however, there was no major increase in the reactive nitrogen species RNS generated in monocytes from both sources. Furthermore, raised levels of superoxide in neutrophils of synovial infiltrate showed a positive correlation with NADPH oxidase activity in synovial fluid. Additionally, as ROS generated in both peripheral blood and synovial infiltrate correlated positively with both DAS 28 and CRP/anti-CCP levels, its measurement can serve as an indirect measure of the degree of infl ammation in patients with RA.