The ladybird homeobox genes are essential for the specification of a subpopulation of neural cells
The ladybird homeobox genes are essential for the specification of a subpopulation of neural cells
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DOI：
10.1016/j.ydbio.2004.02.014
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发表时间：
2004-06-01
影响因子：
2.7
通讯作者：
Jagla, K
中科院分区：
文献类型：
--
作者：
De Graeve, F;Jagla, T;Jagla, K
在果蝇中，神经元和神经胶质细胞是由称为神经母细胞(NBS)的神经前体细胞产生的，可以单独识别。每个NB产生由NB及其后代内基因表达的精确时空控制所指定的特征细胞谱系。在这里，我们证明了同源异型盒基因早瓢虫和瓢虫晚在神经母细胞NB5-3和NB5-6来源的细胞亚群中表达，并且对于它们的正确发育是必不可少的。我们的分析表明，果蝇中的瓢虫，就像它们的脊椎动物同源Lbx1基因一样，在细胞命运指定过程中发挥着重要作用。表达瓢虫的细胞中有NB5-6来源的神经胶质细胞。在瓢虫功能丧失突变体中，Nb5-6衍生的Exit神经胶质细胞缺失，而这些基因的过度表达导致了这种类型的额外神经胶质细胞。此外，在瓢虫功能改变的胚胎中观察到神经根中异常的神经胶质细胞定位和轴突束间距，表明瓢虫基因也可能控制发育中的果蝇腹神经索内的定向细胞运动和细胞与细胞的相互作用。(C)2004 Elsevier Inc.保留所有权利。
In Drosophila, neurons and glial cells are produced by neural precursor cells called neuroblasts (NBs), which can be individually identified. Each NB generates a characteristic cell lineage specified by a precise spatiotemporal control of gene expression within the NB and its progeny. Here we show that the homeobox genes ladybird early and ladybird late are expressed in subsets of cells deriving from neuroblasts NB 5-3 and NB 5-6 and are essential for their correct development. Our analysis revealed that ladybird in Drosophila, like their vertebrate orthologous Lbxl genes, play an important role in cell fate specification processes. Among those cells that express ladybird are NB 5-6-derived glial cells. In ladybird loss-of-function mutants, the NB 5-6-derived exit glial cells are absent while overexpression of these genes leads to supernumerary glial cells of this type. Furthermore, aberrant glial cell positioning and aberrant spacing of axonal fascicles in the nerve roots observed in embryos with altered ladybird function suggest that the ladybird genes might also control directed cell movements and cell-cell interactions within the developing Drosophila ventral nerve cord. (C) 2004 Elsevier Inc. All rights reserved.