INFLUENCE OF ADJUVANTS ON THE QUANTITY, AFFINITY, ISOTYPE AND EPITOPE SPECIFICITY OF MURINE ANTIBODIES
INFLUENCE OF ADJUVANTS ON THE QUANTITY, AFFINITY, ISOTYPE AND EPITOPE SPECIFICITY OF MURINE ANTIBODIES
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DOI：
10.1016/0022-1759(89)90156-7
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发表时间：
1989-07-26
影响因子：
2.2
通讯作者：
ALLISON, AC
中科院分区：
文献类型：
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作者：
KENNEY, JS;HUGHES, BW;ALLISON, AC
将5种佐剂与弗氏S佐剂比较，制备小鼠抗人血清白蛋白和白介素1α的多克隆抗体和单抗。(II-1.Alpha)。检测的参数包括效价亲和力、浓度、同种异型、血清和杂交瘤细胞上清液的表位特异性和中和活性。弗氏S佐剂虽然在血清中产生高滴度和高浓度的抗体，但在诱导具有特殊性质的抗体方面逊于其他佐剂。佐剂Quil A和Al(OH)3/](Thr1)胞壁二肽可诱导出最高亲和力的抗体。Syntex佐剂配方-1(SAF-1)可诱导最高比例的针对HSA的“保护性”IgG2a抗体。所有佐剂，尤其是Quil A和Ribi佐剂系统，在诱导天然和变性人血清白蛋白结合抗体方面均优于弗氏S佐剂；在诱导多克隆抗体和杂交瘤抗体方面，SAF-1优于弗氏S佐剂，可中和IL-1α的生物学活性。这些结果表明，佐剂选择性地、独立地增强不同性质的抗体应答。此外，用合适的佐剂免疫可以优化所需质量的单抗的生产。
Five adjuvants were compared to Freund''s adjuvant for production of mouse polyclonal antibodies and monoclonal antibodies (McAbs) to human serum albumin (HSA) and interleukin-1.alpha. (II-1.alpha.). Parameters examined were titer affinity, concentration, isotype, epitope specificity and neutralizing activity of sera and hybridoma supernatants. Freund''s adjuvant, while producing high titers and concentrations of antibodies in sera, was inferior to other adjuvants for eliciting antibodies with particular qualities. The adjuvants Quil A and Al(OH)3/](Thr1]muramyldipeptide elicited the highest affinity antibodies to HSA. Syntex adjuvant formultion-1 (SAF-1) elicited the highest percentage of ''protective'' IgG2a antibodies to HSA. All adjuvants, particularly Quil A and Ribi adjuvant system, where superior to Freund''s adjuvant in eliciting antibodies which bound native versus denatured HSA. In a comparison of SAF-1 and Freund''s adjuvant, SAF-1 was superior to Freund''s adjuvant in eliciting polyclonal and hybridoma antibodies which neutralized the biological activity of IL-1.alpha.. These results show that adjuvants selectively and independently enhance different qualities of the antibody response. Furthermore, immunization with the appropriate adjuvant can optimize production of McAbs with desired qualities.