Purification and properties of cytochrome c-553, an electron acceptor for formate dehydrogenase of Desulfovibrio vulgaris, Miyazaki.
Purification and properties of cytochrome c-553, an electron acceptor for formate dehydrogenase of Desulfovibrio vulgaris, Miyazaki.
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细胞色素 c-553 的纯化和特性，细胞色素 c-553 是宫崎脱硫弧菌甲酸脱氢酶的电子受体。
DOI：
10.1016/0005-2728(79)90190-7
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发表时间：
1979
期刊：
影响因子：
--
通讯作者：
T. Yagi
中科院分区：
文献类型：
--
作者：
T. Yagi
普通脱硫弧菌（Desulfovibrio vulgaris，宫崎）的细胞色素c-553被纯化至均一。铁型的吸收光谱在553、525、417和317 nm处有四个峰，在280 nm附近有一个平台;铁型的吸收光谱在525、410和360 nm处有三个峰，在280 nm附近有一个平台，在560 nm处有一个肩峰。铁型α峰的毫摩尔吸光度系数为23.9。cytochromec-553的分子量为8000，含有一个血红素。它的等电点是碱性的，在pH7.0时的标准氧化还原电位是−0.26 V。其氨基酸组成独特;它缺乏脯氨酸、异亮氨酸和色氨酸。铁细胞色素c-553不与CO联合收割机结合，也不直接将电子转移到各种氧化还原载体，如黄素核苷酸、亚甲基蓝、靛蓝二磺酸盐、5-甲基吩嗪鎓甲基硫酸盐、1-甲氧基-5-甲基吩嗪鎓甲基硫酸盐、紫精和细胞色素c 3，在甲酸存在下，Cytochromec-553能被该菌的甲酸脱氢酶还原，而在H2存在下，Cytochromec-553不能被氢化酶还原。在存在甲酸盐的情况下，甲酸脱氢酶不会还原细胞色素3。D.甲酸脱氢酶的系统名称。因此，将vulgarisis确定为EC亚类1.2.2.-中的甲酸盐：铁细胞色素-553氧化还原酶。
Cytochromec-553 ofDesulfovibrio vulgaris, Miyazaki, was purified to homogeneity. The absorption spectrum of the ferro form has four peaks at 553, 525, 417 and 317 nm with a plateau near 280 nm, and that of the ferri form has three peaks at 525, 410 and 360 nm with a plateau near 280 nm and a shoulder at 560 nm. The millimolar absorbance coefficient of the α-peak of the ferro form is 23.9. The molecular weight of cytochromec-553 is 8000, and it contains one heme. Its isoelectric point is rather alkaline, and its standard redox potential is −0.26 V at pH 7.0. Its amino acid composition is unique; it lacks proline, isoleucine and tryptophan.Ferrocytochromec-553 does not combine with CO, nor does it transfer electrons directly to various redox carriers such as flavin nucleotides, methylene blue, indigodisulfonate, 5-methylphenazinium methyl sulfate, 1-methoxy-5-methylphenazinium methyl sulfate, viologens and cytochromec3, but is oxidized by ferricyanide or by O2.Cytochromec-553 can be reduced by formate dehydrogenase of this bacterium in the presence of formate, but not by hydrogenase under H2. The formate dehydrogenase does not reduce cytochromec3in the presence of formate. The systematic name for formate dehydrogenase ofD. vulgarisis, therefore, established as formate:ferricytochromec-553 oxidoreductase in EC subclass 1.2.2.—.