Genome-wide RNA-seq analysis of human and mouse platelet transcriptomes
Genome-wide RNA-seq analysis of human and mouse platelet transcriptomes
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DOI：
10.1182/blood-2011-03-339705
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发表时间：
2011-10-06
期刊：
影响因子：
20.3
通讯作者：
Weyrich, Andrew S.
中科院分区：
文献类型：
--
作者：
Rowley, Jesse W.;Oler, Andrew J.;Weyrich, Andrew S.
近交小鼠是研究血小板体内功能的有用工具。尽管如此，小鼠血小板的 mRNA 特征尚不清楚。在这里，我们使用配对末端下一代 RNA 测序 (RNA-seq) 来表征人和小鼠血小板的聚腺苷酸化转录组。我们报告说，RNA-seq 为整个人类和小鼠基因组中表达的 mRNA 提供了前所未有的分辨率。这两个物种之间的转录本表达和丰度通常是保守的。然而，一些 mRNA 在人和小鼠血小板中的表达存在差异。此外，先前描述的小鼠和人类血小板之间的功能差异反映在转录水平的差异上，包括蛋白酶激活受体1、蛋白酶激活受体3、血小板激活因子受体和因子V。这表明RNA-seq是预测小鼠和人类之间血小板功能差异的有用工具。我们的下一代测序分析为人类和小鼠血小板转录组提供了新的见解。测序数据集将有助于设计小鼠止血模型，并成为发现血小板新功能的催化剂。可以在“简介”中找到对数据集的访问。 （血。2011；118(14):e101-e111）
Inbred mice are a useful tool for studying the in vivo functions of platelets. Nonetheless, the mRNA signature of mouse platelets is not known. Here, we use paired-end next-generation RNA sequencing (RNA-seq) to characterize the polyadenylated transcriptomes of human and mouse platelets. We report that RNA-seq provides unprecedented resolution of mRNAs that are expressed across the entire human and mouse genomes. Transcript expression and abundance are of-ten conserved between the 2 species. Several mRNAs, however, are differentially expressed in human and mouse platelets. Moreover, previously described functional disparities between mouse and human platelets are reflected in differences at the transcript level, including protease activated receptor-1, protease activated receptor-3, platelet activating factor receptor, and factor V. This suggests that RNA-seq is a useful tool for predicting differences in platelet function between mice and humans. Our next-generation sequencing analysis provides new insights into the human and murine platelet transcriptomes. The sequencing dataset will be useful in the design of mouse models of hemostasis and a catalyst for discovery of new functions of platelets. Access to the dataset is found in the "Introduction." (Blood. 2011; 118(14):e101-e111)