Effective treatment of osteomyelitis with biodegradable microspheres in a rabbit model
Effective treatment of osteomyelitis with biodegradable microspheres in a rabbit model
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DOI：
10.1097/01.blo.0000126303.41711.a2
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发表时间：
2004-04-01
影响因子：
4.2
通讯作者：
Mikos, AG
中科院分区：
文献类型：
--
作者：
Ambrose, CG;Clyburn, TA;Mikos, AG
生物可降解微球由50%乳酸和50%乙醇酸的高分子量共聚物和抗生素妥布霉素制成。假设微球在妥布霉素的局部递送方面比聚甲基丙烯酸甲酯珠更有效，并且微球不会抑制骨愈合。用金黄色葡萄球菌在40只新西兰白色兔中建立骨髓炎。所有动物在接种后4周对感染的桡骨进行冲洗和清创，并分为5个治疗组：单独清创、单独微球、含妥布霉素的微球加头孢唑林肠外治疗、含妥布霉素的聚甲基丙烯酸甲酯珠加肠外头孢唑林和肠外头孢唑林。治疗4周后处死所有动物。与对照组相比，用微球加胃肠外抗生素治疗的组是唯一在治疗4周后具有显著更高的无细菌动物百分比的组。此外，用微球治疗的动物在缺损处的骨愈合程度高于用骨水泥治疗的动物。在此兔骨髓炎模型中，最有效的治疗是生物可降解微球联合肠外抗生素。
Biodegradable microspheres were manufactured from a high molecular weight copolymer of 50% lactic and 50% glycolic acid and the antibiotic tobramycin. It was hypothesized that the microspheres would be more effective than polymethylmethacrylate beads in the local delivery of tobramycin and that the microspheres would not inhibit bone healing. Osteomyelitis was established in 40 New Zealand White rabbits using Staphylococcus aureus. All animals had irrigation and debridement of the infected radii four weeks after inoculation and were divided into five treatment groups: debridement alone, microspheres alone, microspheres containing tobramycin plus parenteral treatment with cefazolin, polymethylmethacrylate beads containing tobramycin plus parenteral cefazolin, and parenteral cefazolin. All animals were sacrificed after 4 weeks of treatment. The group treated with microspheres plus parenteral antibiotics was the only group to have a significantly higher percentage of animals without bacteria after 4 weeks of treatment when compared with the control group. Additionally, the animals treated with microspheres had a higher degree of bone healing in the defect than the animals treated with bone cement. The most effective treatment was biodegradable microspheres combined with parenteral antibiotic in this rabbit osteomyelitis model.