Macromolecular composition of stress fiber-plasma membrane attachment sites in endothelial cells in situ
Macromolecular composition of stress fiber-plasma membrane attachment sites in endothelial cells in situ
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DOI：
10.1161/01.res.79.5.1000
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发表时间：
1996-11-01
影响因子：
20.1
通讯作者：
Fujiwara, K
中科院分区：
文献类型：
--
作者：
Kano, Y;Katoh, K;Fujiwara, K
应力纤维主要分布在培养细胞的顶端（apical SF）和基部（basal SF）。我们最近的研究表明，根尖SFs以一种类似于基底SFs附着于局灶黏附位点的方式锚定在根尖质膜上。我们建议将这种根尖sf膜附着部位称为“根尖斑块”。“为了研究根尖斑块的大分子组成和内皮细胞（ECs）的局灶黏附，我们通过共聚焦激光扫描和荧光显微镜检查了豚鼠主动脉，并染色了各种针对局灶黏附相关蛋白的抗体。与血流方向平行的基底纤维纤维主要位于细胞的上游。还观察到薄的根尖SFs。用抗血管蛋白、抗talin、抗paxillin或抗纤维连接蛋白受体染色的细胞基底部和表皮部可见点状染色模式，表明内皮细胞原位存在局灶性粘连和表皮斑块。尽管纤维连接蛋白受体存在于根尖斑块。我们的数据表明，在体外和原位细胞中，负责SFPM关联的分子是相同的。我们的结果似乎支持了SF系统参与流体机械力的传感和/或信号转导的假设。
Stress fibers (SFs) are present along the apical (apical SF) and the basal (basal SF) portions of cultured cells. We have recently shown that apical SFs are anchored to the apical plasma membrane (PM) in a manner similar to how basal SFs are attached to focal adhesion sites. We propose calling such apical SF-membrane attachment sites ''apical plaques.'' To study the macromolecular composition of the apical plaque and the focal adhesion in endothelial cells (ECs) in situ, we examined by confocal laser scanning and fluorescence microscopy guinea pig aortae stained with various antibodies against focal adhesion-associated proteins. Basal SFs oriented parallel to the blood flow direction were mainly located in the upstream half of the cell. Thin apical SFs were also observed. Spotty staining patterns were observed in the basal and the epical portions of cells stained with anti-vinculin, anti-talin, anti-paxillin, or anti-fibronectin receptor, indicating the presence of focal adhesions and epical plaques in ECs in situ. Although fibronectin receptors were present in the apical plaque. fibronectin was not detected on the apical cell surface, Our data suggest that the molecules responsible for the SFPM association are the same between in vitro and in situ cells. Our results appear to support a hypothesis that the SF system is involved in sensing and/or signal transduction of fluid mechanical forces.