Biochemistry, cytogenetics and bioaccumulation in silver catfish (Rhamdia quelen) exposed to different thorium concentrations.
Biochemistry, cytogenetics and bioaccumulation in silver catfish (Rhamdia quelen) exposed to different thorium concentrations.
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DOI：
10.1016/j.aquatox.2008.05.003
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发表时间：
2008-07
影响因子：
4.5
通讯作者：
L. M. Correa;D. Kochhann;A. G. Becker;M. A. Pavanato;S. Llesuy;V. Loro;A. Raabe;M. Mesko;É. M. Flores;V. Dressler;B. Baldisserotto
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文献类型：
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作者：
L. M. Correa;D. Kochhann;A. G. Becker;M. A. Pavanato;S. Llesuy;V. Loro;A. Raabe;M. Mesko;É. M. Flores;V. Dressler;B. Baldisserotto
本研究的目的是通过肌肉的生化参数（糖原、葡萄糖、乳酸、蛋白质和氨）评估钍（Th）生物累积对银鲶鱼（Rhamdia quelen）代谢的影响。此外，还分析了鳃、肝和肌肉组织中的脂质过氧化水平（TBARS）、过氧化氢酶（CAT）和谷胱甘肽-S-转移酶（GST）。通过评估红细胞核异常来研究细胞遗传学参数。将银鲶鱼幼鱼暴露于不同的水中Th水平（以μgL−1为单位）：0（对照）、25.3±3.2、69.2±2.73、209.5±17.6和608.7±61.1中15天。积累最高Th水平的器官是鳃和皮肤。水性Th浓度的增加对应于鳃、肝脏、皮肤和肾脏中Th水平的逐渐增加，其中在鳃和皮肤中积累最高。肌肉中的代谢中间体因钍暴露而改变，但没有发现明确的关系。该物种肝脏和肌肉组织中的CAT和GST活性表明，酶活性在最低Th水平下受到刺激，在较高水平（主要在608.7μgL−1）下受到抑制。细胞遗传学检测的结果有助于这一假设，因为在暴露于 69.2 和 209.5μgL−1Th 的幼体中发现血液样本具有较高的毒性。
The objective of this study was to evaluate the effect of thorium (Th) bioaccumulation on the metabolism of silver catfish (Rhamdia quelen) through biochemical parameters of the muscle (glycogen, glucose, lactate, protein, and ammonia). In addition, lipidic peroxidation levels (TBARS), catalase (CAT) and glutathione-S-transferase (GST) in the gills and in hepatic and muscular tissues were also analyzed. Cytogenetic parameters were studied through the evaluation of nuclear abnormalities in red blood cells. Silver catfish juveniles were exposed to different waterborne Th levels (in μgL−1): 0 (control), 25.3±3.2, 69.2±2.73, 209.5±17.6, and 608.7±61.1 for 15 days. The organs that accumulated the highest Th levels were the gills and skin. The increase of waterborne Th concentration corresponded to a progressive increase of Th levels in the gills, liver, skin and kidneys, with the highest accumulation in the gills and skin. Metabolic intermediates in the muscle were altered by Th exposure, but no clear relationship was found. CAT and GST activities in the hepatic and muscular tissues of this species suggest that the enzymatic activities can be stimulated at the lowest Th levels and inhibited at the higher levels (mainly in 608.7μgL−1). The results of the cytogenetic assay contribute to this hypothesis because the higher toxicity in blood samples was found in juveniles exposed to 69.2 and 209.5μgL−1Th.