Identification of a single major genetic locus controlling the resistance to lymphocystis disease in Japanese flounder (Paralichthys olivaceus)
Identification of a single major genetic locus controlling the resistance to lymphocystis disease in Japanese flounder (Paralichthys olivaceus)
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DOI：
10.1016/j.aquaculture.2005.11.024
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发表时间：
2006-04-28
期刊：
影响因子：
4.5
通讯作者：
Okamoto, Nobuaki
中科院分区：
文献类型：
--
作者：
Fuji, Kanako;Kobayashi, Kazunobu;Okamoto, Nobuaki
日本牙鲆（Paralichthys olivaceus）是一种重要的经济食用鱼类，在日本、韩国和中国等亚洲国家广泛养殖。我们选择研究淋巴囊肿病（LD），因为它已在这些国家广泛传播并严重损害了养鱼场。 LD 是虹彩病毒引起的感染。患有 LD 的日本比目鱼在皮肤、鳍和/或嘴上会出现典型的肥大细胞，称为淋巴囊肿细胞。带有淋巴囊肿细胞的鱼因为丑陋而失去了商业价值。口腔中的淋巴囊肿细胞会妨碍正常进食，在最坏的情况下可能导致饥饿。因此，这种疾病对农民来说代价高昂。目前尚无针对 LD 的治疗方法，也没有市售疫苗。在本研究中，我们尝试通过连锁分析来鉴定LD抗性基因座（LD-R），因为它可能有助于建立LD抗性菌株的工作。我们使用50个微卫星标记在日本牙鲆中寻找与LD抗性相关的基因座。在通过易感雄性与（易感x抗性）杂种雌性杂交产生的回交后代（n = 13 6）中进行连锁测试。在日本牙鲆遗传连锁图谱的连锁群（LG）15上检测到LD-R的一个主要位点。在 LG15 上，微卫星标记 (Poli.9-8TUF) 解释了 136 名筛选个体中总表型变异的 50%。因此，它可以被认为是标记辅助育种的候选位点，以提高LD抗性的选择效率。 (c) 2005 Elsevier B.V 保留所有权利。
Japanese flounder (Paralichthys olivaceus) is an economically important fish as a food, and has been widely cultured in Asian countries such as Japan, Korea and China. We chose to study lymphocystis disease (LD) because it has become widely spread in these countries and seriously damaged fish farms. LD is an Iridovirus-induced infection. Japanese flounder affected with LD develop characteristically hypertrophied cells, called lymphocystis cells, on skin, fins and/or mouth. Fish with lymphocystis cells lose commercial value because they are ugly. Lymphocystis cells on the mouth prevent proper feeding and in the worst case can lead to starvation. The disease is thus costly to farmers. There are presently no treatment for LD nor commercially available vaccines. In this study, we tried to identify the LD-resistance locus (LD-R) by linkage analysis because it may facilitate the effort for the establishment of LD-resistance strains.We used 50 microsatellite markers to search for a locus associated with resistance to LD in Japanese flounder. Linkage tests were conducted in a backcross progeny (n = 13 6) produced by crossing a susceptible male with a (susceptible x resistant) hybrid female.One major locus for LD-R was detected on linkage group (LG) 15 of the Japanese flounder genetic linkage map. On LG15, a microsatellite marker (Poli.9-8TUF) explained 50% of the total phenotypic variation in the 136 individuals screened. Therefore, it can be considered as a candidate locus for marker-assisted breeding in order to enhance selective efficiency for LD resistance. (c) 2005 Elsevier B.V All rights reserved.