Expression of OCT4 in the primary germ cell tumors and thymoma in the mediastinum
Expression of OCT4 in the primary germ cell tumors and thymoma in the mediastinum
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DOI：
10.1097/00129039-200609000-00004
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发表时间：
2006-09-01
影响因子：
1.6
通讯作者：
Lin, Pyng-Jing
中科院分区：
文献类型：
--
作者：
Jung, Shih-Ming;Chu, Pao-Hsien;Lin, Pyng-Jing
原发性生殖细胞肿瘤（GCT）和胸腺瘤都位于前纵隔。先前的研究已经假设八聚体结合转录因子（octamer binding transcription factor，OCT 4）是在多能胚胎生殖细胞中表达的核转录因子。本研究检测了OCT 4在GCT和起源于纵隔的胸腺瘤中的表达。一项回顾性研究纳入了1983年至2005年期间进行的46例连续GCT患者，以及22例连续纵隔胸腺瘤患者，其肿瘤已手术切除。46例原发于纵隔的巨细胞瘤包括畸胎瘤（n = 27; 58.7%），腺瘤（n = 10; 21.7%），卵黄囊瘤（n = 6; 13%），胚胎性癌（n = 1; 2.1%）和混合GCT 2例（4%），1例为畸胎瘤和卵黄囊瘤，1例为畸胎瘤、卵黄囊瘤和卵巢癌;胸腺瘤22例，包括世界卫生组织A型AB型4例（18.2%），B1型6例（27.3%），B2型4例（13.6%），B3型5例（22.7%）。用苏木精和伊红染色以及OCT 4抗体检查每个肿瘤。所有10例乳腺癌、1例胚胎癌和1例混合型GCT均为OCT 4阳性。另一方面，22例胸腺瘤、6例卵黄囊瘤、27例畸胎瘤和1例无卵黄囊瘤成分的混合性GCT均为OCT 4免疫阴性。我们的结论是，免疫组化与抗体OCT 4是一个有用的诊断工具，在确定的恶性肿瘤和原发性胚胎癌的GCT起源于纵隔。
Primary germ cell tumors (GCTs) and thymoma are both located in the anterior mediastinum. A previous study has postulated that octamer binding transcription factor (OCT4) is a nuclear transcription factor that is expressed in pluripotent embryonic germ cells. This study examined OCT4 expression in GCTs and thymoma originating from the mediastinum. A retrospective study included 46 consecutive patients with GCTs conducted between 1983 and 2005, and 22 consecutive thymoma in the mediastinum whose tumors had been surgically excised. The 46 primary GCTs in mediastinum included teratoma (n = 27; 58.7%), seminoma (n = 10; 21.7%), yolk sac tumor (n = 6; 13%), embryonal carcinoma (n = 1; 2.1%), and mixed GCTs (n = 2; 4%; one consisted of teratoma and yolk sac tumor, and the other teratoma, yolk sac tumor, and seminoma); and 22 thymoma including World Health Organization type A (n = 3, 13.6%), type AB (n = 4, 18.2%), type B1 (n = 6, 27.3%), type B2 (n = 4, 13.6%), and type B3 (n = 5, 22.7%). Each tumor was examined with hematoxylin and eosin staining, and with antibodies to OCT4. All 10 seminoma cases, 1 embryonal carcinoma case, and 1 mixed GCT case containing seminoma were immunopositive for OCT4. On the other hand, the 22 thymoma, 6 yolk sac tumor, 27 teratomas, and I case with mixed GCT without component of seminoma were immunonegative for OCT4. We conclude that immunostaining with antibodies to OCT4 is a useful diagnostic tool in the identification of seminomas and primary embryonal carcinomas in GCTs originating from the mediastinum.