A phosphoethanolamine-modified glycosyl diradylglycerol in the polar lipids of Clostridium tetani
A phosphoethanolamine-modified glycosyl diradylglycerol in the polar lipids of Clostridium tetani
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DOI：
10.1194/jlr.m004788
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发表时间：
2010-07-01
影响因子：
6.5
通讯作者：
Goldfi, Howard
中科院分区：
文献类型：
--
作者：
Johnston, Norah C.;Aygun-Sunar, Semra;Goldfi, Howard
采用二维薄层色谱法、ESI质谱法和核磁共振波谱法研究了厌氧细菌破伤风梭菌（Clostridium tetani）的极性脂质。除了菌株E88的亲本菌株ATCC 10779外，大多数菌株的主要脂质为磷脂酰乙醇胺、磷脂酰甘油、心磷脂和n -乙酰氨基葡萄糖二烷基甘油的磷脂酰原和二酰和四酰化种。该菌株含有相同的二酰化和四酰化种，但不含缩醛原。所有菌株都含有一种新的n -乙酰氨基葡萄糖二烷基甘油衍生物，其中磷酸乙醇胺单元连接在糖的6'位置，通过选择性p -31解耦，h -1检测NMR差谱来判断。n -乙酰氨基葡萄糖（GlcNAc）残基可能与二二基甘油部分的3位相连，并且具有β -端粒结构。jlr质谱法在前体磷脂酸和磷脂酰丝氨酸中检测到很少的磷脂酰原成分，这与厌氧梭菌磷脂组装后期由二酰化磷脂形成的观点一致。——约翰斯顿，n.c.， S. aygan - sunar，管正洲，A. A. Ribeiro， F. Daldal， C. R. H. Raetz和H. Goldfine。破伤风梭菌极性脂质中一种磷酸乙醇胺修饰的糖基二烷基甘油。[j] .油脂杂志。2010。51: 1953 - 1961。
The polar lipids of the anaerobic bacterium Clostridium tetani, the causative agent of tetanus, have been examined by two-dimensional thin layer chromatography, ESI mass spectrometry, and NMR spectroscopy. Plasmalogen and di- and tetra-acylated species of phosphatidylethanolamine, phosphatidylglycerol, cardiolipin, and N-acetylglucosaminyl diradylglycerol were the major lipids present in most strains examined except for strain ATCC 10779, the parent of strain E88, the first C. tetani strain to have its genome sequenced. This strain contained the same di- and tetra-acylated species but did not contain plasmalogens. All strains contained a novel derivative of N-acetylglucosaminyl diradylglycerol in which a phosphoethanolamine unit is attached to the 6'-position of the sugar, as judged by selective P-31-decoupled, H-1-detected NMR difference spectroscopy. The N-acetylglucosamine (GlcNAc) residue is presumably linked to the 3-positon of the diradylglycerol moiety, and it has the beta-anomeric configuration.jlr Very little plasmalogen component was detected by mass spectrometry in the precursors phosphatidic acid and phosphatidylserine, consistent with the idea that plasmalogens are formed from diacylated phospholipids at a late stage of phospholipid assembly in anaerobic clostridia.-Johnston, N. C., S. Aygun-Sunar, Z. Guan, A. A. Ribeiro, F. Daldal, C. R. H. Raetz, and H. Goldfine. A phosphoethanolamine-modified glycosyl diradylglycerol in the polar lipids of Clostridium tetani. J. Lipid Res. 2010. 51: 1953-1961.