CALCIUM-DEPENDENT PROLONGED EFFECTS ON MELANOPHORES OF [4-NORLEUCINE, 7-D-PHENYLALANINE]-ALPHA-MELANOTROPIN
CALCIUM-DEPENDENT PROLONGED EFFECTS ON MELANOPHORES OF [4-NORLEUCINE, 7-D-PHENYLALANINE]-ALPHA-MELANOTROPIN
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DOI：
10.1126/science.6973820
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发表时间：
1981-01-01
期刊：
影响因子：
56.9
通讯作者：
HRUBY, VJ
中科院分区：
文献类型：
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作者：
HADLEY, ME;ANDERSON, B;HRUBY, VJ
单次注射促黑素类似物[4-去甲亮氨酸，7- d -苯丙氨酸]-。将-促黑素注入蛙类（Rana pipiens），导致蛙类皮肤近乎最大程度的变黑，持续至少6周。注射天然激素。-促黑素或类似物[Nle4]-。-促黑素也会使皮肤变黑，但这种效果只持续几天。[Nle4， D-Phe7]- α注射青蛙皮肤的形态学观察。-促黑素显示，在整个6周期间，真皮和表皮的黑色素细胞都是分散的。体外[Nle4， D-Phe7]-. α。-促黑素还会导致卡罗莱纳蜥蜴皮肤长时间变黑。在没有促黑素的情况下，先前用类似物变暗的皮肤可以通过从培养培养基中去除Ca来变浅，然后可以通过添加Ca来重新变暗。该循环可以在不添加促黑素的情况下无限重复。这些结果证明了Ca在受体信号转导中的作用以及[Nle4， D-Phe7]- α的长期生物学效应。-促黑素在从实验培养基中去除后很长一段时间。
A single injection of the melanotropin analog [4-norleucine, 7-D-phenylalanine]-.alpha.-melanotropin into frogs (Rana pipiens) caused near maximum darkening of the skins of the frogs for at least 6 wk. Injections of the natural hormone .alpha.-melanotropin or of the analog [Nle4]-.alpha.-melanotropin also caused darkening, but this effect lasted only a few days. Morphological examination of the skins of frogs injected with [Nle4, D-Phe7]-.alpha.-melanotropin revealed that both dermal and epidermal melanophores were dispersed during the entire 6-wk period. In vitro [Nle4, D-Phe7]-.alpha.-melanotropin also causes prolonged darkening of the skin of the lizard Anolis carolinensis. In the absence of the melanotropin, skins previously darkened with the analog could be lightened by removal of Ca from the incubation medium but could then be redarkened by adding Ca. The cycle could be repeated indefinitely without addition of melanotropin. These results demonstrate the role of Ca in receptor signal transduction and the prolonged biological effects of [Nle4, D-Phe7]-.alpha.-melanotropin long after its removal from the assay medium.