Studies of nucleotide sequences in tobacco mosaic virus ribonucleic acid. IV. Use of aniline in stepwise degradation.
Studies of nucleotide sequences in tobacco mosaic virus ribonucleic acid. IV. Use of aniline in stepwise degradation.
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烟草花叶病毒核糖核酸核苷酸序列的研究。
DOI：
10.1021/bi00872a034
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发表时间：
1966
期刊：
影响因子：
2.9
通讯作者：
H. Fraenkel
中科院分区：
文献类型：
--
作者：
A. Steinschneider;H. Fraenkel
材料和方法从伊士曼有机化工公司购买苯胺，重新蒸馏，然后保存在深色瓶子里，冷藏。用水稀释1：33(v/v)，用浓盐酸将溶液调节到所需的pH值。本溶液新鲜配制而成。M.Gordon博士和W.Wacker博士亲切地向我们介绍了从E.Cali中提取的碱性磷酸酶--磷酸单酯酶的制备。用本实验室的标准方法提取TMV-RNA、[32P]TMV-RNA、[，4C]TMV-RNA和[3 2P]5‘-核苷酸(Fraenkel-Conrat和Singer，1962；Sugiyama和Fraenkel-Conrat，1961)。高碘酸氧化、[14C]氨基脲处理和沉淀分析如前一篇论文所述(Steinschneider和Fraenkel-Conrat，
Materials and Methods Aniline was purchased from Eastman Organic Chemicals, redistilled, and stored in a dark bottle in the cold. It was diluted 1: 33 (v/v) with water and the solution adjusted to the desired pH by means of concentrated HC1. This solution was prepared fresh for use.Preparations of phosphomonoesterase, 1 the alkaline phosphatase from E. cali, were kindly given us by Drs. M. Gordon and W. Wacker. TMV-RNA,[32P] TMV-RNA,[, 4C] TMV-RNA, and [3 2P] 5'-nucleotides were isolated by the standard methods of this laboratory (Fraenkel-Conrat and Singer, 1962; Sugiyama and Fraenkel-Conrat, 1961). Periodate oxidation,[14C] semicarbazide treatment, and sedimentation analysis were performed as in the preceding paper (Steinschneider and Fraenkel-Conrat,