A two-generation analysis of pollen pool genetic structure in flowering dogwood, Cornus florida (Cornaceae), in the Missouri Ozarks.
A two-generation analysis of pollen pool genetic structure in flowering dogwood, Cornus florida (Cornaceae), in the Missouri Ozarks.
复制标题
对密苏里州奥沙克地区山茱萸（山茱萸科）花粉库遗传结构的两代分析。
DOI：
10.3732/ajb.92.2.262
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发表时间：
2005
影响因子：
3
通讯作者：
R. Westfall
中科院分区：
文献类型：
--
作者：
V. Sork;P. Smouse;V. Apsit;R. Dyer;R. Westfall
人为景观变化会扰乱基因流动。作为密苏里州奥扎克森林生态系统项目的一部分，本研究通过比较皆伐、选择性砍伐和未砍伐制度之间的花粉库结构（Φ(st)），研究造林实践是否影响昆虫授粉物种 Cornus florida L. 中花粉介导的基因运动，并期望未砍伐制度中花粉运动应最少。使用来自 6 个地点（每个地点范围为 266 至 527 公顷）的 150 个种子亲本（43 个为明确种子亲本，74 个为选择性种子亲本，33 个为对照地点）的 1500 株幼苗样本，每株 10 株，用称为 TwoGener 的花粉池结构方法分析了 8 个同位素酶位点（Smouse 等人，2001 年；Evolution 55：260-271）。该分析表明，花粉库结构在清晰区域（Φ（C）= 0.090，P < 0.001）比未切割区域（Φ（U）= 0.174，P < 0.001）要少，并且有选择性切割中间区域（Φ（S）= 0.125，P < 0.001）。这些估计转化为，在明确切割区域 (N(ep) = 5.56) 和选择性切割区域 (N(ep) = 4.00) 中，比未切割区域 (N(ep) = 2.87) 更有效的花粉供体 (N(ep))。我们证明 Φ(C) ≤ Φ(S) ≤ Φ(U)，其中 Φ(C) 显着小于 Φ(U) (P < 0.034)。研究结果表明，只要保留足够数量的种子亲本以提供充分的繁殖并避免母亲有效数量的遗传瓶颈，造林管理可能不会对花粉亲本的有效数量产生负面影响，从而不会对佛罗里达州山茱萸的遗传多样性产生负面影响。
Anthropogenic landscape change can disrupt gene flow. As part of the Missouri Ozark Forest Ecosystem Project, this study examined whether silvicultural practices influence pollen-mediated gene movement in the insect-pollinated species, Cornus florida L., by comparing pollen pool structure (Φ(st)) among clear-cutting, selective cutting, and uncut regimes with the expectation that pollen movement should be least in the uncut regime. Using a sample of 1500 seedlings-10 each from 150 seed parents (43 in clear-cut, 74 in selective, and 33 in control sites) from six sites (each ranging from 266 to 527 ha), eight allozyme loci were analyzed with a pollen pool structure approach known as TwoGener (Smouse et al., 2001; Evolution 55: 260-271). This analysis revealed that pollen pool structure was less in clear-cut (Φ(C) = 0.090, P < 0.001) than in uncut areas (Φ(U) = 0.174, P < 0.001), with selective-cut intermediate (Φ(S) = 0.125, P < 0.001). These estimates translate into more effective pollen donors (N(ep)) in clear-cut (N(ep) = 5.56) and selective-cut (N(ep) = 4.00) areas than in uncut areas (N(ep) = 2.87). We demonstrate that Φ(C) ≤ Φ(S) ≤ Φ(U), with Φ(C) significantly smaller than Φ(U) (P < 0.034). The findings imply that, as long as a sufficiently large number of seed parents remain to provide adequate reproduction and to avoid a genetic bottleneck in the effective number of mothers, silvicultural management may not negatively affect the effective number of pollen parents, and hence subsequent genetic diversity in Cornus florida.
影响因子：
3.3
作者：
L. Excoffier;P. Smouse;J. Quattro
通讯作者：
L. Excoffier;P. Smouse;J. Quattro