Comparative proteomic analysis of silkworm fat body after knocking out fibroin heavy chain gene: a novel insight into cross-talk between tissues
Comparative proteomic analysis of silkworm fat body after knocking out fibroin heavy chain gene: a novel insight into cross-talk between tissues
复制标题
丝蛋白重链基因敲除后蚕脂肪体的比较蛋白质组学分析：组织间串扰的新见解
DOI：
10.1007/s10142-015-0461-0
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发表时间：
2015-09-01
影响因子：
2.9
通讯作者：
Xia, Qingyou
中科院分区：
文献类型：
--
作者：
Chen, Quanmei;Ma, Zhengang;Xia, Qingyou
组织间串扰在生物体发育过程中起着重要作用，但昆虫组织间串扰的研究却很少。我们的前期研究表明，敲除丝素重链基因（BmFib-H）后，蛹体重增加，而该基因在家蚕丝腺中特异表达。因此，该突变体是研究组织间串扰的良好材料。家蚕幼虫期的脂肪体被认为是贮存蛹发育所需营养物质的物质。本研究对家蚕Fib-H基因敲除的家蚕Moriline（FGKO）及其野生型大造进行了5龄5日龄脂肪体的比较蛋白质组学研究。这些结果表明，丝腺中的单个基因敲除引发了脂肪体代谢途径的大规模变化。FGKO脂肪体中参与糖酵解/糖异生、磷酸戊糖途径和甘氨酸-丝氨酸生物合成途径的蛋白质水平下调。与此相反，许多参与蛋白质合成的蛋白质，包括核糖体蛋白，真核生物翻译起始因子和延伸因子的丰度上调。此外，FGKO脂肪体中糖原和蛋白质的浓度大大增加。这些发现为深入了解家蚕丝腺与脂肪体之间的相互作用提供了新的视角，丝腺与脂肪体之间的相互作用可以调节FGKO脂肪体中营养物质的再分配，从而导致蛹重的增加。
Cross-talk between tissues plays key roles in development of organisms; however, there are few researches on cross-talk between tissues in insects. Our previous studies showed that the pupal body weight was elevated after knocking out the fibroin heavy chain gene (BmFib-H), whereas the gene specifically expressed in silk glands of silkworm. Hence, the mutant is a good material for studying the cross-talk between tissues. It is considered that the fat body of silkworm during larval stage is used to store nutrients for pupal development. Herein, comparative proteomic of fat body on the 5th day of fifth instar was performed betweenBmFib-Hgene knock-outBombyx moriline (FGKO) and its wide-typeDazao. These results revealed that a single gene knock-out in silk gland triggered large-scale metabolic pathways changes in fat body. The levels of proteins involved in glycolysis/gluconeogenesis, pentose phosphate pathway, and glycine-serine biosynthetic pathway were down-regulated in the FGKO fat body. In contrast, the abundances of many proteins participating in protein synthesis, including ribosomal proteins, eukaryotic translation initiation factor, and elongation factor, were up-regulated. Moreover, the concentrations of glycogen and proteins in the FGKO fat body were greatly increased. These findings provided a novel insight into the cross-talk between silk gland and fat body in silkworm, and the presence of cross-talk between silk gland and fat body could regulate the redistribution of nutrients in the FGKO fat body leading to the increase of the pupal weight.