Calcium regulatory action of endogenous rat calcitonin demonstrated by passive immunization with calcitonin antibodies.
Calcium regulatory action of endogenous rat calcitonin demonstrated by passive immunization with calcitonin antibodies.
复制标题
通过降钙素抗体被动免疫证明内源性大鼠降钙素的钙调节作用。
DOI：
10.1210/endo-107-5-1320
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发表时间：
1980
期刊：
影响因子：
4.8
通讯作者：
D. Kalu
中科院分区：
文献类型：
--
作者：
B. Roos;M. Yoon;S. Cutshaw;D. Kalu
通过结合免疫学技术、生物测定和腺体消融来评估降钙素 (CT) 在小鼠生理学中的钙调节作用。用中和CT抗体对大鼠进行被动免疫，导致血浆钙立即但短暂地增加，其时间过程与甲状旁腺切除动物中观察到的相似。在白天禁食并在夜间（1700-0700小时）进食的动物中，通过甲状腺甲状旁腺切除术或CT抗体被动免疫急剧降低内源性CT（<100pg/ml）导致餐前（1600小时）血浆钙的增加比餐后免疫反应性CT和血浆钙（已知的CT促分泌剂）之间的负相关更大。在这些严格喂养的动物中，还观察到餐前（1600 小时）免疫反应性和生物活性 CT 急剧上升，以及血浆钙的昼夜节律。循环CT增加的开始发生在进食开始之前，并且与血浆钙的每日最低点同时或不久之前。这些发现证实 CT 在小鼠生理学中具有主要的钙调节作用。更具体地说，他们证明：1）甲状腺甲状旁腺切除术后血浆钙的增加具体是由于CT的损失，2）被动免疫是评估内源性CT作用的可行技术，3）CT在钙调节中的作用不能仅限于防止餐中和餐后血钙增加，4）除了钙以外的因素必须参与非进食状态下CT分泌的控制。
The calcium regulatory role of calcitonin (CT) in murine physiology was evaluated with a combination of immunological techniques, bioassays, and gland ablation. Passive immunization of rats with neutralizing CT antibodies caused an immediate but transient increase in plasma calcium with a time course similar to that observed in thyroparathyroidectomized animals. In animals fasted during the day and fed at night (1700-0700 h), acutely decreasing endogenous CT (< 100 pg/ml) by thyroparathyroidectomy or by passive immunization with CT antibodies resulted in a greater increase in plasma calcium in the preprandial (1600 h) than in the postprandial inverse correlation between immunoreactive CT and plasma calcium, a known secretagogue for CT. In these animals on a restricted feeding schedule, a sharp preprandial (1600 h) rise in immunoreactive and bioactive CT and a circadian rhythm in plasma calcium were also observed. The onset of the increase in circulating CT preceded the onset of feeding and coincided with or shortly preceded the daily nadir of plasma calcium. These findngs establish that CT has a primary calcium regulatory role in murine physiology. More specifically, they demonstrate that 1) the postthyroparathyroidectomy increase in plasma calcium is due specifically to the loss of CT, 2) passive immunization is a feasible technique for evaluating the actions of endogenous CT, 3) the role of CT in calcium regulation cannot be restricted to that of preventing prandial and postprandial increase in blood calcium, and 4) factors other than calcium must be involved in the control of CT secretion in the nonfed state.