Development, validation and clinical application of a LC-MS/MS method for the simultaneous quantification of hydroxychloroquine and its active metabolites in human whole blood
Development, validation and clinical application of a LC-MS/MS method for the simultaneous quantification of hydroxychloroquine and its active metabolites in human whole blood
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DOI：
10.1016/j.jpba.2014.07.009
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发表时间：
2014-11-01
影响因子：
3.4
通讯作者：
Bourgogne, Emmanuel
中科院分区：
文献类型：
--
作者：
Soichot, Marion;Megarbane, Bruno;Bourgogne, Emmanuel
建立了一种快速、灵敏、特异的液相色谱-串联质谱法同时测定人全血中羟氯喹（HCQ）及其3种主要代谢物的方法。使用100 μ L的样品体积，对于所有四种化合物，该测定在25-2000 ng/mL的动态范围内呈线性（R-2 > 0.99），并且在适当的样品稀释后，适合于测定高达20，000 ng/mL的升高的HCQ浓度。试验间和试验内精密度为
A rapid, sensitive and specific method using liquid chromatography coupled to tandem mass spectrometry was developed for the simultaneous quantification of hydroxychloroquine (HCQ) and its three major metabolites in human whole blood. The assay, using a sample volume of 100 mu L, was linear in a dynamic 25-2000 ng/mL range (R-2 > 0.99) for all four compounds and suitable for the determination of elevated HCQ concentrations up to 20,000 ng/mL, after appropriate sample dilution. Inter- and intra-assay precisions were