Comparative analysis of 87,000 expressed sequence tags from the fumonisin-producing fungus Fusarium verticillioides
Comparative analysis of 87,000 expressed sequence tags from the fumonisin-producing fungus Fusarium verticillioides
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DOI：
10.1016/j.fgb.2005.06.001
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发表时间：
2005-10-01
影响因子：
3
通讯作者：
Whitelaw, CA
中科院分区：
文献类型：
--
作者：
Brown, DW;Cheung, F;Whitelaw, CA
串珠镰刀菌（有性态为藤仓赤霉）是一种在世界范围内危害玉米的病原菌，它能产生伏马菌素，这是一类真菌毒素，与多种动物疾病以及人类癌症有关。在本研究中，我们试图鉴定影响伏马菌素产生和/或植物 - 真菌相互作用的真菌基因。我们从9个不同的cDNA文库中获得了超过87,000个表达序列标签，这些标签对应11,119条独特序列，估计代表了基因基因组组成的80%。对这些文库的比较分析表明，伏马菌素基因簇中的所有15个基因都存在差异表达。此外，还鉴定出9个候选的伏马菌素调控基因以及一些可能在植物 - 真菌相互作用中起作用的基因。对来自5个不同文库的700多个FUM基因转录本的分析为具有未剪接内含子以及具有可变3'剪接位点的剪接内含子的转录本提供了证据。这些可变剪接形式的丰富程度以及在参与单一代谢物家族生物合成的基因中发现它们的频率，还有它们的差异表达，都表明它们可能具有生物学功能。最后，对一个与FUM12和FUM13之间的基因组序列比对的EST的分析，为FUM基因簇中一个先前未鉴定的基因（FUM20）提供了证据。由爱思唯尔公司出版。
Fusarium verticillioides (teleomorph Gibberella moniliformis) is a pathogen of maize worldwide and produces fumonisins, a family of mycotoxins that have been associated with several animal diseases as well as cancer in humans. In this study, we sought to identify fungal genes that affect fumonisin production and/or the plant-fungal interaction. We generated over 87,000 expressed sequence tags from nine different cDNA libraries that correspond to 11, 119 unique sequences and are estimated to represent 80% of the genomic complement of genes. A comparative analysis of the libraries showed that all 15 genes in the fumonisin gene cluster were differentially expressed. In addition, nine candidate fumonisin regulatory genes and a number of genes that may play a role in plant-fungal interaction were identified. Analysis of over 700 FUM gene transcripts from five different libraries provided evidence for transcripts with unspliced introns and spliced introns with alternative 3' splice sites. The abundance of the alternative splice forms and the frequency with which they were found for genes involved in the biosynthesis of a single family of metabolites as well as their differential expression suggest they may have a biological function. Finally, analysis of an EST that aligns to genomic sequence between FUM12 and FUM13 provided evidence for a previously unidentified gene (FUM20) in the FUM gene cluster. Published by Elsevier Inc.