Effect of selenite, vitamin E and N,N'-diphenyl-p-phenylenediamine on liver organic solvent-soluble lipofuscin pigments in mice.
Effect of selenite, vitamin E and N,N'-diphenyl-p-phenylenediamine on liver organic solvent-soluble lipofuscin pigments in mice.
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亚硒酸盐、维生素E和N,N-二苯基对苯二胺对小鼠肝脏有机溶剂可溶性脂褐质色素的影响。
DOI：
10.1093/jn/114.9.1582
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发表时间：
1984
期刊：
影响因子：
--
通讯作者：
B. Z. Menken
中科院分区：
文献类型：
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作者：
A. S. Csallany;L. Su;B. Z. Menken
本实验研究了不同剂量维生素E和N，N '-二苯基对苯二胺（DPPD）对小鼠肝脏有机溶剂可溶性脂褐素（OLP）积累和谷胱甘肽过氧化物酶（GSH-Px）活性的影响。四组16只雌性断奶小鼠，每组喂食维生素E缺乏饲料、补充30 mg/kg或300 mg/kg维生素E（作为RRR-alpha-生育酚乙酸酯）的饲料或补充30 mg/kg DPPD的饲料。每种日粮含硒0.05ppm。在5个月大时，每个饮食组中的8只动物在其饮用水中补充额外的0.1 ppm Se（亚硒酸钠）。其余动物在9个月的实验期内饲喂其原始饲料。亚硒酸盐补充导致OLP浓度和GSH-Px活性的显着增加，在小鼠肝脏喂养维生素E或DPPD补充饮食。正常水平的维生素E和DPPD（30 mg/kg）不足以防止亚硒酸盐的氧化作用;然而，10倍于正常水平的维生素E（300 mg/kg）显着抑制这种氧化作用。
The present experiment was designed to investigate the effect of selenium (Se) supplementation, as sodium selenite, on organic solvent-soluble lipofuscin pigment (OLP) accumulation and glutathione peroxidase (GSH-Px) activity in the livers of mice fed varying levels of vitamin E or N,N'-diphenyl-p-phenylenediamine (DPPD). Four groups of 16 female, weanling mice each were fed either a vitamin E-deficient diet, a diet supplemented with 30 mg/kg or 300 mg/kg vitamin E (as RRR-alpha-tocopheryl acetate), or a diet supplemented with 30 mg/kg DPPD. Each diet contained 0.05 ppm Se. At 5 months of age, eight animals from each dietary group were supplemented with an additional 0.1 ppm Se, as sodium selenite, in their drinking water. The remaining animals were fed their original diets through the 9-month experimental period. Selenite supplementation resulted in a significant increase in OLP concentration and GSH-Px activity in the liver of mice fed vitamin E- or DPPD-supplemented diets. Normal levels of vitamin E and DPPD (30 mg/kg) were not sufficient to protect against the oxidative effects of selenite; however, 10 times the normal level of vitamin E (300 mg/kg) markedly suppressed this oxidative effect.