Local expression of CYP19A1 and CYP19A2 in developing and adult killifish (Fundulus heteroclitus)
Local expression of CYP19A1 and CYP19A2 in developing and adult killifish (Fundulus heteroclitus)
复制标题
DOI：
10.1016/j.ygcen.2007.05.018
复制
发表时间：
2008-01-15
影响因子：
2.7
通讯作者：
Willett, Kristine L.
中科院分区：
文献类型：
--
作者：
Dong, Wu;Willett, Kristine L.
P450芳香化酶（CYP 19）是甾体激素合成途径的末端酶，催化雄激素转化为雌激素。异基底鱼与其他硬骨鱼一样，表达两个CYP 19基因，CYP 19 A1和CYP 19 A2。用原位杂交法检测了CYP 19 s在胚胎发育过程中的表达。在90 dpf（受精后天数）鱼和成鱼中，CYP 19 A1在早期I期卵母细胞（初级生长期）的卵质中表达。CYP 19 A1的表达定位于I期晚期（卵黄发生前期）和II期（卵黄发生期）卵泡的卵泡细胞层中，但到了III期（早期成熟卵泡），CYP 19 A1的表达定位于卵黄被膜中。总体而言，CYP 19 A1卵母细胞膜表达逐渐下降，从晚期I的最高表达到IV期的不可检测水平。CYP 19 A2在4、6、8、10、14 dpf胚胎、90 dpf鱼苗和成鱼脑的下丘脑中表达最高。与雄性相比，雌性嗅球中的CYP 19 A2表达更高。除脑外，6-14 dpf胚胎的肾上腺/肾细胞中也存在强CYP 19 A2信号。这项工作建立了CYP 19在底中的定位和组成型表达，然后可以将其与环境污染物引起的CYP 19 A1和CYP 19 A2表达的潜在破坏和生理后果进行比较。(c)2007爱思唯尔公司All rights reserved.
P450 aromatase (CYP19) is the terminal enzyme in the steroidogenic pathway and catalyzes the conversion of androgens to estrogens, Fundulus heteroclitus like other teleosts, express two CYP19 genes, CYP19A1 and CYP19A2. The expression of CYP19s in Fundulus was measured by in situ hybridization throughout development. In 90 dpf (day post-fertilization) fish and adult fish, CYP19A1 was expressed in the ooplasm of early stage I oocytes (primary growth stage). Expression of CYP19A1 was localized in the follicle cell layer of late stage I (previtellogenic stage) and stage II (vitellogenic stage) follicles, but by stage III (early maturational follicles) CYP19A1 expression was localized in the vitelline envelope. Overall, CYP19A1 oocyte membrane expression gradually declined from highest expression at late stage I to nondetectable levels by stage IV. Highest expression of CYP19A2 was detected in the brain including the hypothalamus from 4, 6, 8, 10, 14 dpf embryos, 90 dpf fry fish and adult fish brain. In females compared to males, there was higher CYP19A2 expression in olfactory bulb. In addition to the brain, there was strong CYP19A2 signal in adrenal/kidney cells in 6-14 dpf embryos. This work establishes the localization and constitutive expression of CYP19s in Fundulus which can then be compared with potential disruption of CYP19A1 and CYP19A2 expression and physiological consequences caused by environmental contaminants. (c) 2007 Elsevier Inc. All rights reserved.