Organization of Cerebral Projections to Identified Cerebellar Zones in the Posterior Cerebellum of the Rat
Organization of Cerebral Projections to Identified Cerebellar Zones in the Posterior Cerebellum of the Rat
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DOI：
10.1523/jneurosci.0857-12.2012
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发表时间：
2012-08-08
影响因子：
5.3
通讯作者：
Ruigrok, Tom J. H.
中科院分区：
文献类型：
--
作者：
Suzuki, Lucia;Coulon, Patrice;Ruigrok, Tom J. H.
小脑连接利用了哺乳动物大脑中两个最大的纤维束，即，大脑和内侧小脑脚。旨在阐明这一重要连接的组织的神经解剖学方法受到其多突触性质、其组成部分的复杂组织以及传统示踪剂对精确定位注射的依赖性的阻碍。为了克服这些问题，我们使用狂犬病病毒（RV）作为逆行跨神经元示踪剂。将RV与霍乱毒素β亚单位（CTb）同时注射到18只雄性Wistar大鼠小脑皮质的选定区域。48 - 50小时的存活时间导致RV的一级和二级标记与CTb的一级标记组合。CTb标记的神经元在下橄榄的分布建立了注射部位的区域性身份。用这种方法，我们可以观察到神经元的皮质分布，这些神经元是小脑投射到特定小脑位点的神经元，结果表明，这种分布与被注射的小脑小叶的特性有关。当注射同一小叶的不同区域时，出现更微妙的变化。与C2/D区相比，小叶VIII的C1区从体感皮层接收更突出的投射。横向定位的D区接收来自大脑皮层更多吻侧区域的信息。第七小叶的蚓部接收来自压后皮质和眶额皮质的显著输入。不同的注射部位也会导致连接的偏侧性差异。
The cerebrocerebellar connection makes use of two of the largest fiber tracts in the mammalian brain, i.e., the cerebral and medial cerebellar peduncles. Neuroanatomical approaches aimed to elucidate the organization of this important connection have been hindered by its multisynaptic nature, the complex organization of its components, and the dependency of conventional tracers on precisely placed injections. To overcome these problems, we used rabies virus (RV) as a retrograde transneuronal tracer. RV was injected simultaneously with cholera toxin beta subunit (CTb) into selected areas of the cerebellar cortex of 18 male Wistar rats. A survival time of 48 - 50 h resulted in first- and second-order labeling of RV in combination with first- order labeling of CTb. The distribution of CTb-labeled neurons in the inferior olive established the zonal identity of the injection site. In this way, it was possible to examine the cortical distribution of neurons from which disynaptic cerebrocerebellar projections to specific cerebellar loci originate.The results show that this distribution covaries with the identity of the injected cerebellar lobule. More subtle changes were present when different zones of the same lobule were injected. The C1 zone of lobule VIII receives a more prominent projection from the somatosensory cortex compared with the C2/D zones. The laterally positioned D zones receive information from more rostral regions of the cerebral cortex. The vermis of lobule VII receives a prominent input from the retrosplenial and orbitofrontal cortices. Different injection sites also result in differences in laterality of the connections.