The ethylene response factor SmERF6 co-regulates the transcription of SmCPS1 and SmKSL1 and is involved in tanshinone biosynthesis in Salvia miltiorrhiza hairy roots
The ethylene response factor SmERF6 co-regulates the transcription of SmCPS1 and SmKSL1 and is involved in tanshinone biosynthesis in Salvia miltiorrhiza hairy roots
复制标题
乙烯反应因子SmERF6共同调控SmCPS1和SmKSL1的转录并参与丹参毛状根中丹参酮的生物合成
DOI：
10.1007/s00425-018-2884-z
复制
发表时间：
2018-07-01
期刊：
影响因子：
4.3
通讯作者：
Liang, Zongsuo
中科院分区：
文献类型：
--
作者：
Bai, Zhenqing;Li, Wenrui;Liang, Zongsuo
SmERF6识别细胞核中SmCPS1和SmKSL1启动子的GCC-box，调节丹参毛状根中丹参酮的生物合成。丹参酮是丹参中的重要药用成分，以其在医学上的用途而闻名。然而，丹参酮生物合成的转录因子调控尚不清楚。在这里，我们分离并鉴定了丹参ERF家族中的一个转录因子SmERF6，它是通过两个关键丹参酮合成基因（SmKSL1和SmCPS1）的启动子从丹参cDNA文库中筛选得到的。该因子调节丹参酮的生物合成。该基因在根中高表达，并对乙烯处理有反应。 SmERF6 通过直接结合 SmKSL1 和 SmCPS1 启动子的乙烯响应元件 (GCC-box) 并激活其转录来调节丹参酮生物合成。毛状根中 SmERF6 的过度表达增加了丹参酮的积累，而通过 RNAi 沉默 SmERF6 导致丹参酮含量降低。此外，丹参酮的积累与丹参中总酚酸和黄酮类化合物的含量保持稳态。这些发现阐明了SmERF6如何直接共同调节SmCPS1和SmKSL1的转录并调节丹参酮合成以加速丹参中丹参酮积累的代谢流。
The SmERF6, which recognizes the GCC-box of SmCPS1 and SmKSL1 promoter in nucleus, regulates the tanshinone biosynthesis in Salvia miltiorrhiza hairy roots. Tanshinone, an important medicinal ingredient in Salvia miltiorrhiza, is best known for its use in medicine. However, the transcription factor regulation of tanshinone biosynthesis is unclear. Here, we isolated and identified a transcription factor in the ERF family of S. miltiorrhiza, SmERF6, which was screened from an S. miltiorrhiza cDNA library by the promoters of two key tanshinone synthesis genes (SmKSL1 and SmCPS1); this factor regulated tanshinone biosynthesis. The gene was highly expressed in the root and responded to ethylene treatment. SmERF6 modulated tanshinone biosynthesis by directly binding to an ethylene-responsive element (GCC-box) of the SmKSL1 and SmCPS1 promoters and activating their transcription. Overexpression of SmERF6 in the hairy roots increased their tanshinone accumulation, and SmERF6 silencing by RNAi led to a lower tanshinone content. Furthermore, tanshinone accumulation maintained homeostasis with the total phenolic acid and flavonoid contents in S. miltiorrhiza. These findings elucidated how SmERF6 directly co-regulates the transcription of SmCPS1 and SmKSL1 and modulates tanshinone synthesis to accelerate the metabolic flux of tanshinone accumulation in S. miltiorrhiza.