Adipose-derived stem cells from the brown bear (Ursus arctos) spontaneously undergo chondrogenic and osteogenic differentiation in vitro
Adipose-derived stem cells from the brown bear (Ursus arctos) spontaneously undergo chondrogenic and osteogenic differentiation in vitro
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DOI：
10.1016/j.scr.2011.03.003
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发表时间：
2011-07-01
影响因子：
1.2
通讯作者：
Frobert, Ole
中科院分区：
文献类型：
--
作者：
Fink, Trine;Rasmussen, Jeppe G.;Frobert, Ole
在洞穴中，冬眠的棕熊不会出现组织萎缩或器官损伤，尽管几乎没有体力活动。间充质干细胞在棕熊组织修复和再生中可能发挥重要作用。我们的目的是确定是否可以从野生斯堪的纳维亚棕熊中回收脂肪组织来源的干细胞（ASCs），并表征其分化潜力。4月中旬，野生棕熊离开洞穴后7-10天被固定，然后进行脂肪组织活检。从6只熊中回收了ASC，并显示其能够在单层培养中进行脂肪生成和骨生成，在颗粒培养中进行软骨生成。值得注意的是，当在单层培养的标准细胞培养基中生长时，来自一岁鸽的ASC自发地形成被软骨沉积物包围的骨样结节，这表明分化成成骨和软骨生成谱系。这种能力随着年龄的增长而逐渐丧失。这是第一项证明棕熊干细胞恢复和生长的研究，也是第一次报道在没有特异性诱导剂的情况下，ASCs自发形成骨和软骨细胞外基质的特征。这些发现可能对冬眠棕熊作为研究废用性骨质疏松症的模型的使用产生影响。(C)2011爱思唯尔有限公司版权所有。
In the den, hibernating brown bears do not develop tissue atrophy or organ damage, despite almost no physical activity. Mesenchymal stem cells could play an important role in tissue repair and regeneration in brown bears. Our objective was to determine if adipose tissue-derived stem cells (ASCs) can be recovered from wild Scandinavian brown bears and characterize their differentiation potential. Following immobilization of wild brown bears 7-10 days after leaving the den in mid-April, adipose tissue biopsies were obtained. ASCs were recovered from 6 bears, and shown to be able to undergo adipogenesis and osteogenesis in monolayer cultures and chondrogenesis in pellet cultures. Remarkably, when grown in standard cell culture medium in monolayer cultures, ASCs from yearlings spontaneously formed bone-like nodules surrounded by cartilaginous deposits, suggesting differentiation into osteogenic and chondrogenic lineages. This ability appears to be lost gradually with age. This is the first study to demonstrate stem cell recovery and growth from brown bears, and it is the first report of ASCs spontaneously forming extracellular matrix characteristic of bone and cartilage in the absence of specific inducers. These findings could have implications for the use of hibernating brown bears as a model to study disuse osteoporosis. (C) 2011 Elsevier B.V. All rights reserved.