Reconsolidation and update of morphine-associated contextual memory in mice
Reconsolidation and update of morphine-associated contextual memory in mice
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DOI：
10.1016/j.nlm.2016.02.015
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发表时间：
2016-04-01
影响因子：
2.7
通讯作者：
Santos-Junior, Jair Guilherme
中科院分区：
文献类型：
--
作者：
Escosteguy-Neto, Joao Carlos;Varela, Patricia;Santos-Junior, Jair Guilherme
药物成瘾可以看作是一种不断被检索和重新巩固的病态记忆。由于药物滥用发生在不同的情况下，可以认为再巩固在记忆更新中起作用。有一致的证据支持再巩固在药物滥用引起的情景记忆的强度和维持中的作用。然而，这个角色在内存更新中并没有得到很好的确立。本研究的目的是评估记忆更新的再巩固过程。C57BL6小鼠接受吗啡诱导的条件位置偏好（CPP）范式。根据CPP结果，根据再暴露的环境特征和第二次CPP测试将动物分为不同的实验组。在原始情境下的再暴露对记忆维持很重要，在离散情境变化下的再暴露导致记忆更新，尽管原始记忆保持不变。有趣的是，环己亚胺，一种蛋白质合成抑制剂，在我们的方案中有不同的结果。当在离散的环境变化下再次暴露时，环己亚胺治疗在再次暴露后阻止了记忆更新，而没有改变记忆维持。当在原始环境下再次暴露时，只有两次随后的环己亚胺注射（3和6小时）破坏了后来的CPP表达。考虑到巩固和再巩固过程中蛋白质合成的时间窗口，这些发现表明，根据我们的研究方案中的上下文特征，重新暴露可能触发这两种现象。此外，当检索时出现新信息时，再巩固在记忆更新中起关键作用。(C) 2016 Elsevier Inc.版权所有。
Drug addiction can be viewed as a pathological memory that is constantly retrieved and reconsolidated. Since drug abuse takes place in different contexts, it could be considered that reconsolidation plays a role in memory updating. There is consistent evidence supporting the role of reconsolidation in the strength and maintenance of contextual memories induced by drugs of abuse. However, this role is not well established in memory update. The purpose of the current study was to assess the reconsolidation process over memory update. C57BL6 mice were subjected to a morphine-induced, conditioned place preference (CPP) paradigm. Based on CPP results, animals were divided into distinct experimental groups, according to the contextual characteristics of the re-exposure and a second CPP Test. Re-exposure in the original context was important for memory maintenance and re-exposure under discrete contextual changes resulted in memory updating, although original memory was maintained. Interestingly, cycloheximide, an inhibitor of protein synthesis, had different outcomes in our protocol. When the re-exposure was done under discrete contextual changes, cycloheximide treatment just after re-exposure blocked memory updating, without changes in memory maintenance. When re-exposure was done under the original context, only two subsequent cycloheximide injections (3 and 6 h) disrupted later CPP expression. Considering the temporal window of protein synthesis in consolidation and reconsolidation, these findings suggest that re-exposure, according to the contextual characteristics in our protocol, could trigger both phenomena. Furthermore, when new information is present on retrieval, reconsolidation plays a pivotal role in memory updating. (C) 2016 Elsevier Inc. All rights reserved.