A polymorphism within the equine CRISP3 gene is associated with stallion fertility in Hanoverian warmblood horses
A polymorphism within the equine CRISP3 gene is associated with stallion fertility in Hanoverian warmblood horses
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DOI：
10.1111/j.1365-2052.2007.01594.x
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发表时间：
2007-06-01
期刊：
影响因子：
2.4
通讯作者：
Leeb, T.
中科院分区：
文献类型：
--
作者：
Hamann, H.;Jude, R.;Leeb, T.
公马的繁殖力具有很高的经济重要性，特别是对于大型繁殖组织和种马。通过包括与性状相关的分子遗传标记，可以改进早期阶段关于生育特征和种马选择的育种计划。富含马半胱氨酸的分泌蛋白(CRIPS)的编码基因是很有希望的候选基因，因为之前的研究表明，CRIPS对雄性动物的受精能力起到了作用。我们之前已经确定了这三个马CRISP基因的特征，并在CRISP1基因中发现了一个非同义的多态。在本研究中，我们报告了CRISP2基因的一个非同义多态和CRISP3基因的四个非同义多态。对107匹汉诺威种公马进行了6个CRISP多态的基因分型。利用种马受精记录，分析了脆性多态与育性性状的关系。采用三种统计模型分析单突变、基因分型和单倍型对基因多态的影响。导致E208K氨基酸替换的CRISP3 AJ459965：C.+622G>；A SNP与种马的繁殖力显著相关。CRISP3 c.+622G>；A SNP杂合子公马的受精率低于纯合子公马(P=0.0234)。据估计，这些种马的每个周期的妊娠率比在这个位置纯合子的种马低7%。
Fertility of stallions is of high economic importance, especially for large breeding organisations and studs. Breeding schemes with respect to fertility traits and selection of stallions at an early stage may be improved by including molecular genetic markers associated with traits. The genes coding for equine cysteine-rich secretory proteins (CRISPs) are promising candidate genes because previous studies have shown that CRISPs play a role in the fertilising ability of male animals. We have previously characterised the three equine CRISP genes and identified a non-synonymous polymorphism in the CRISP1 gene. In this study, we report one non-synonymous polymorphism in the CRISP2 gene and four non-synonymous polymorphisms in the CRISP3 gene. All six CRISP polymorphisms were genotyped in 107 Hanoverian breeding stallions. Insemination records of stallions were used to analyse the association between CRISP polymorphisms and fertility traits. Three statistical models were used to evaluate the influence of single mutations, genotypes and haplotypes of the polymorphisms. The CRISP3 AJ459965:c.+622G > A SNP leading to the amino acid substitution E208K was significantly associated with the fertility of stallions. Stallions heterozygous for the CRISP3 c.+622G > A SNP had lower fertility than homozygous stallions (P = 0.0234). The pregnancy rate per cycle in these stallions was estimated to be similar to 7% lower than in stallions homozygous at this position.