Integrating a Microwave Resonator and a Microchannel with an Immunochromatographic Strip for Stable and Quantitative Biodetection.
Integrating a Microwave Resonator and a Microchannel with an Immunochromatographic Strip for Stable and Quantitative Biodetection.
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DOI：
10.1021/acsami.9b02087
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发表时间：
2019-03
影响因子：
9.5
通讯作者：
Hong Zhou;Cheng Yang;Donglin Hu;Shaoxu Dou;Xindan Hui;Feng Zhang;Cong Chen;Ming Chen;Ya Yang;X. Mu
中科院分区：
文献类型：
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作者：
Hong Zhou;Cheng Yang;Donglin Hu;Shaoxu Dou;Xindan Hui;Feng Zhang;Cong Chen;Ming Chen;Ya Yang;X. Mu
免疫层析试条因其简单、快速和成本效益高而在各个领域成为一种有效的诊断工具。然而，用于初步筛选的典型试条只能提供定性或半定量的结果，而通过将不同种类的纳米颗粒作为生物标志物来进行定量检测的常见解决方案仍然不能彻底解决这一问题。在这里，我们试图通过集成低成本的薄膜兼容方形裂环谐振器和结构设计的柔性微通道与柔性条来应对这一挑战。实验证明，金黄色葡萄球菌定量检测纸条的检出限和灵敏度分别达到0.784 ng/mL和10.214 MHz/(ng/mL)。LOD水平比肉眼测得的色基试条高约63倍，稳定性比荧光试条高约18倍。这项工作不仅可以为金黄色葡萄球菌或其他分子的定量检测提供强有力的诊断工具，而且为实现生物分子的电场检测、生物传感器的系统级集成以及便携式诊断设备的开发提供了新的途径。
An immunochromatographic strip is an effective diagnostic tool in various fields because of its simplicity, rapidity, and cost-effectiveness. However, typical strips for preliminary screening provide only qualitative or semiquantitative results, and common solutions for quantitative detection by incorporating different kinds of nanoparticles as biomarkers still do not solve this problem thoroughly. Here, we try to tackle this challenge by integrating low-cost membrane-compatible square split-ring resonators and structure-design-flexible microchannels with flexible strips. We experimentally demonstrate that the limit of detection (LOD) and sensitivity of the strip for quantitative detection of Staphylococcus aureus reach 0.784 ng/mL and 10.214 MHz/(ng/mL), respectively. The LOD level is about 63 times higher than that of the color-based strip determined by the naked eye, and the stability is about 18 times higher than that of the fluorescent strip. This work could not only provide a powerful diagnosis tool for the quantitative detection of S. aureus or other molecules but also deliver new avenues for achieving electric field detection of biomolecules, system-level integration of biosensors, and the development of portable diagnostic devices.