Characterisation of the mating-type locus of the plant pathogenic ascomycete Leptosphaeria maculans
Characterisation of the mating-type locus of the plant pathogenic ascomycete Leptosphaeria maculans
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DOI：
10.1007/s00294-003-0391-6
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发表时间：
2003-08-01
期刊：
影响因子：
2.5
通讯作者：
Howlett, BJ
中科院分区：
文献类型：
--
作者：
Cozijnsen, AJ;Howlett, BJ
描述了含有植物病原子囊菌斑球菌交配型基因座的区域的核苷酸序列。 MAT1-1基因全长1,368 bp，编码441个氨基酸的预测蛋白，具有45 bp内含子。 MAT1-2基因长1,246 bp，编码397个氨基酸的预测蛋白，具有55 bp内含子。后一个基因位于一个小的开放阅读框（32 个氨基酸）下游 334 bp，其中四个氨基酸的位置与 MAT1-2 基因的高迁移率基团结合域中的位置相同。 DNA 裂解酶和后期促进复合体基因位于 MAT 基因的 3' 端，而异旋旋孢菌中的 ORF1 基因和 GTPase 激活蛋白则位于 MAT 的 5' 端。确定了 L. maculans MAT 基因座内部和侧翼基因的转录模式。 MAT 转录本的长度约为基因长度的两倍。 ORF1 转录本在 MAT1-1 分离株中为 1.2 kb，在 MAT1-2 分离株中为 1.0 kb；和探针交叉杂交较弱。针对 L. maculans 开发了一种具有三种核苷酸引物的交配型 PCR 测定方法。
The nucleotide sequences of regions containing the mating-type locus of the plant-pathogenic ascomycete Leptosphaeria maculans are described. The MAT1-1 gene is 1,368 bp, encoding a predicted protein of 441 amino acids, with a 45-bp intron. The MAT1-2 gene is 1,246 bp, encoding a predicted protein of 397 amino acids, with a 55-bp intron. This latter gene is 334 bp downstream of a small open reading frame (32 amino acids) with four amino acids in identical positions to those in the high mobility group binding domain of the MAT1-2 genes. The DNA lyase and anaphase promoting complex genes are 3' of the MAT gene, whilst a gene denoted ORF1 in Cochliobolus heterostrophus and the GTPase activating protein are present 5' of MAT. The transcriptional patterns of genes within and flanking the L. maculans MAT locus are determined. The MAT transcripts are about twice the length of the gene. The ORF1 transcript is 1.2 kb in the MAT1-1 isolate and 1.0 kb in the MAT1-2 isolate; and probes cross-hybridise weakly. A mating-type PCR assay with three nucleotide primers is developed for L. maculans.