Orthotopic murine model of head and neck squamous cell carcinoma
Orthotopic murine model of head and neck squamous cell carcinoma
复制标题
DOI：
10.1016/s0001-6519(05)78579-4
复制
发表时间：
2005-01-01
影响因子：
1
通讯作者：
Chiara, M. D.
中科院分区：
文献类型：
--
作者：
Cabanillas, R.;Secades, P.;Chiara, M. D.
目的：建立一种可靠的能再现头颈部鳞状细胞癌（HNSCC）行为的动物模型。该模型将有助于我们了解这些肿瘤的致瘤性和进展的分子机制，以及评估新的治疗方法。材料和方法：将来自人声门鳞状细胞癌的细胞系经口内和粘膜下注射给20只裸鼠nu/nu。结果如下：90%的小鼠发生局部侵袭性鳞状细胞癌，侵入周围的肌纤维并进入疏松的结缔组织结构。所有肿瘤均呈神经周围生长。18只小鼠中有4只（22%）显示骨破坏，22%血管浸润。所有标本中肿瘤细胞均侵入淋巴管，100%的小鼠发生区域淋巴结转移。所有动物均未发生血行转移。结论：我们提出了一种HNSCC的模型，在许多方面类似于人类。
Purpose: To develop a reliable animal model able to reproduce the behavior of head and neck squamous cell carcinomas (HNSCC). This model should facilitate our understanding of the molecular mechanisms of tumorigenicity and progression of these tumors, as well as the evaluation of novel therapies. Material and methods: 20 nude mice nu/nu were injected intraorally and submucosally with a cell line derived from a human squamous cell carcinoma of the glottis. Results: 90% of the mice developed locally agressive squamous cell carcinomas, invading the surrounding muscle fibers and into loose connective tissue structures. All the tumors showed perineural growth. Four (22%) of the 18 mice showed bone destruction, and 22% vascular invasion. Tumor cells invaded lymphatic vessels in all the specimens, and 100% of the mice developed regional lymph node metastases. None of the animals developed haematogenous metastases. Conclusions: We present a metastasing model of HNSCC that resembles its human counterpart in many aspects.