Evaluation of breast tissue with confocal strip-mosaicking microscopy: a test approach emulating pathology-like examination
Evaluation of breast tissue with confocal strip-mosaicking microscopy: a test approach emulating pathology-like examination
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DOI：
10.1117/1.jbo.22.3.034002
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发表时间：
2017-03-01
影响因子：
3.5
通讯作者：
Murray, Melissa P.
中科院分区：
文献类型：
--
作者：
Abeytunge, Sanjee;Larson, Bjorg;Murray, Melissa P.
Confocal microscopy is an emerging technology for rapid imaging of freshly excised tissue without the need for frozen-or fixed-section processing. Initial studies have described imaging of breast tissue using fluorescence confocal microscopy with small regions of interest, typically 750 x 750 mu m(2). We present exploration with a microscope, termed confocal strip-mosaicking microscope (CSM microscope), which images an area of 2 x 2 cm(2) of tissue with cellular-level resolution in 10 min of excision. Using the CSM microscope, we imaged 34 fresh, human, large breast tissue specimens from 18 patients, blindly analyzed by a board-certified pathologist and subsequently correlated with the corresponding standard fixed histopathology. Invasive tumors and benign tissue were clearly identified in CSM strip-mosaic images. Thirty specimens were concordant for image-to-histopathology correlation while four were discordant. (C) The Authors. Published by SPIE under a Creative Commons Attribution 3.0 Unported License. Distribution or reproduction of this work in whole or in part requires full attribution of the original publication, including its DOI.
