A simple system for automated two-dimensional electrophoresis: applications to genetic testing.
A simple system for automated two-dimensional electrophoresis: applications to genetic testing.
复制标题
一种简单的自动二维电泳系统：在基因检测中的应用。
DOI：
10.1089/gte.1998.2.67
复制
发表时间：
1998
期刊：
影响因子：
--
通讯作者：
Vijg,J
中科院分区：
文献类型：
--
作者：
Dhanda,RK;Smith,WM;Scott,CB;Eng,C;Vijg,J
二维基因扫描（TDGS）是一种基于PCR扩增DNA片段根据大小和碱基对序列进行二维电泳分离的突变检测方法。为了便于在没有人工干扰的情况下进行二维电泳，在现有垂直电泳仪器的顶部设计了一个简单的三室单元。通过对遗传性非息肉病性结肠癌综合征易感基因之一hMLH1进行完整的二维突变扫描测定，证明了该系统在基因测试中的应用。
Two-dimensional gene scanning (TDGS) is a method for mutation detection based on two-dimensional electrophoretic separation of PCR-amplified DNA fragments according to size and base pair sequence. To facilitate two-dimensional electrophoresis without manual interference, a simple three-chamber unit was designed on top of an existing vertical electrophoresis instrument. Application of this system to genetic testing is demonstrated by the performance of a complete two-dimensional mutational scanning assay of one of the hereditary nonpolyposis colon cancer syndrome susceptibility genes,hMLHl.