Accurate dispensing system for single oocytes using air ejection
Accurate dispensing system for single oocytes using air ejection
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DOI：
10.1063/1.4824394
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发表时间：
2013-09-01
期刊：
影响因子：
3.2
通讯作者：
Arai, Fumihito
中科院分区：
文献类型：
--
作者：
Feng, Lin;Sun, Yiling;Arai, Fumihito
在这项研究中，我们提出了一种新的方法来提高单卵母细胞分配的成功率，并调查分配的卵母细胞的后续活力。我们使用一对放置在微流控芯片中的电容传感器来检测卵母细胞，并在微通道中定制设计了特殊的缓冲区，以减慢流速并降低作用于卵母细胞的液压。在缓冲区中，由等间距微柱形成的半圆形隔室用于将卵母细胞阻挡在分配喷嘴孔处。最后，卵母细胞被气流喷射到培养阵列。所开发的微流体系统的新特征是成功率的显著提高伴随着卵母细胞存活率的缺乏变化（如通过比较分配程序之前和之后的存活率所评估的）。通过使用该设备，我们实现了高度准确的单卵母细胞分配过程，成功率为100%。无论是否分配卵母细胞，卵母细胞存活率约为70%。新提出的系统具有操作速度快和潜在的二维微图案化的使用的优点。(C)2013 AIP Publishing LLC.
In this study, we propose a new approach to increase the success rate of single-oocyte dispensing and investigate the subsequent viability of the dispensed oocytes. We used a pair of capacitance sensors placed in a microfluidic chip to detect the oocyte, and custom-designed a special buffer zone in the microchannel to decelerate the flow velocity and reduce the hydraulic pressure acting on the oocyte. In the buffer zone, a semicircular bay, formed by equally spaced micropillars, is used to stop the oocyte at the dispensing nozzle hole. Finally, the oocyte is ejected by airflow to the culture array. The novel feature of the developed microfluidic system is that the extraordinary improvement in success rate is accompanied by a lack of change in oocyte survival rate (as assessed by a comparison of survival rates before and after the dispensing procedure). By using this device, we achieved a highly accurate single-oocyte dispensing process with a success rate of 100%. The oocyte survival rate is approximately 70%, regardless of whether or not the oocyte is dispensed. The newly proposed system has the advantages of high operation speed and potential usage for two-dimensional micropatterning. (C) 2013 AIP Publishing LLC.