A finite representation model for an asynchronous culture of E. coli
A finite representation model for an asynchronous culture of E. coli
复制标题
大肠杆菌异步培养的有限表示模型
DOI：
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发表时间：
1984
影响因子：
3.8
通讯作者：
M. Shuler
中科院分区：
文献类型：
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作者：
M. Domach;M. Shuler
本文描述了一个模拟E.大肠杆菌通过使用大的，但有限数量的代表性的单细胞。通过调节负责隔膜形成的酶的活性，在单细胞水平上发生非同步产生和维持。这种调制引入了周期时间的不精确性，并且不需要将任何新参数引入单细胞模型。基于与实验的比较，合理的预测是可能的细胞干重的变化，在指数增长和turbidostat冲洗，和整体恒化器的细胞产量和细胞数量的变化，葡萄糖浓度，和细胞大小分布的恒化器受到一个步骤的变化，在稀释率。此外，细胞RNA含量和大小之间的相关性被预测为当恒化器停留时间在初始高葡萄糖浓度的条件下减少时的惯性效应。模型的有限性及其解决方案所施加的限制进行了讨论。
A computer model is described which models an asynchronous population of E. coli by using a large, but finite number of representative single cells. Asynchrony generation and maintenance occurs at the single cell level by modulating the activity of an enzyme responsible for septum formation. Such modulation introduces cycle time imprecision and does not require the introduction of any new parameters into the single‐cell model. Based on comparisons to experiment, reasonable predictions are possible for changes of cellular dry weight during exponential growth and turbidostat washout, and overall chemostat cell yields and changes in cell number, glucose concentration, and cell size distribution for a chemostat subject to a step change in dilution rate. Additionally, a correlation between cell RNA content and size is predicted as is an inertial effect when chemostat residence time is decreased under conditions of initially high glucose concentrations. Limitations imposed by the model's finite nature and their solutions are discussed.