Vascular development in embryoid bodies: quantification of transgenic intervention and antiangiogenic treatment
Vascular development in embryoid bodies: quantification of transgenic intervention and antiangiogenic treatment
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DOI：
10.1007/s10456-007-9076-y
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发表时间：
2007-01-01
期刊：
影响因子：
9.8
通讯作者：
Charnock-Jones, D. Stephen
中科院分区：
文献类型：
--
作者：
Evans, Amanda Lisabeth;Bryant, James;Charnock-Jones, D. Stephen
越来越清楚的是，血管发育的研究最好在整个组织环境的背景下考虑，因为体内内皮细胞与其他细胞类型密切相互作用。小鼠胚状体已被用作血管网络早期发育的模型，并适合于遗传操作和可溶性调节剂治疗。然而，量化这些复杂的三维结构中的形态变化是具有挑战性的。在本文中，我们描述的协议，培养胚状体的大规模研究血管的发展与方法，以量化的变化时所看到的抗血管生成剂或内皮细胞特异性转基因的介绍。
It has become increasingly clear that the investigation of vascular development is best considered in the context of a whole tissue environment since in vivo endothelial cells interact closely with other cell types. Murine embryoid bodies have been used as a model for the early development of a vascular network and are amenable to genetic manipulation and treatment with soluble modulators. However, quantifying morphological changes in these complex three-dimensional structures is challenging. In this paper we describe protocols to culture embryoid bodies on a large scale to study vascular development together with methods to quantify changes seen when antiangiogenic agents or endothelial cell-specific transgenes are introduced.