Regulation of Hormone-Related Genes in Ericerus pela (Hemiptera: Coccidae) for Dimorphic Metamorphosis
Regulation of Hormone-Related Genes in Ericerus pela (Hemiptera: Coccidae) for Dimorphic Metamorphosis
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白蜡虫（半翅目：球虫科）激素相关基因对二态变态的调控
DOI：
10.1093/jisesa/iez092
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发表时间：
2019-10-14
影响因子：
2.2
通讯作者：
Chen Hang
中科院分区：
文献类型：
--
作者：
Liu PengFei;Wang Weiwei;Chen Hang
摘要昆虫激素调节变态，包括导致两性异形的变态。利用RNA-Seq技术，我们发现白色二龄雄虫（SM）与二龄雌虫（SF）和一龄雄虫（FM）相比，分别有5，968和8，620个差异表达的转录本。SM中参与旧组织凋亡和新组织重建的基因表达水平显著增强，而SF主要是几丁质等合成代谢基因的表达水平增强。推测二龄幼虫是性二型变态的发育起点。同时，在保幼激素（JH）代谢途径中，CYP 15 A1和JH酯酶（JHE）差异表达;在20-羟基蜕皮激素（20 E）代谢途径中，CYP 307 A1、CYP 314 A1和CYP 18 A1差异表达。在SM中，CYP 307 A1和CYP 314 A1的表达水平显著升高，而CYP 18 A1的表达水平显著降低;在SF中，上述基因的表达水平与SM相反。RNA-seq的表达趋势与qRT-PCR的表达水平一致，其中7个高度相关（R ≥ 0.610），4个中度相关（0.588 ≥ R ≥ 0.542）。
Abstract Insect hormones regulate metamorphosis including that leading to sexual dimorphism. Using RNA-Seq, we discovered that the second-instar male larva (SM) of the white wax insect, Ericerus pela, have 5,968 and 8,620 differentially expressed transcripts compared with the second-instar female larva (SF) and the first-instar male larva (FM), respectively. The expression levels of genes involved in the apoptosis of old tissues and the reconstruction of new ones in the SM significantly enhanced, while the SF mainly has enhanced expression levels of anabolic genes such as chitin. We predicted that the second-instar larvae are the developmental origin of sexual dimorphic metamorphosis. Meanwhile, in the juvenile hormone (JH) metabolic pathway, CYP15A1 and JH esterase (JHE) are differentially expressed; and in the 20-hydroxyecdysone (20E) metabolic pathway, CYP307A1, CYP314A1, and CYP18A1 are differentially expressed. In the SM, the expression levels of CYP307A1 and CYP314A1 are significantly increased, whereas the expression level of CYP18A1 is significantly decreased; in the SF, the expression levels of the above genes are opposite to that of the SM. Expression trends of RNA-seq is consistent with the expression level of qRT–PCR, and seven of them are highly correlated (R ≥ 0.610) and four are moderately correlated (0.588 ≥ R ≥ 0.542).