Templated agarose scaffolds support linear axonal regeneration
Templated agarose scaffolds support linear axonal regeneration
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DOI：
10.1089/ten.2006.12.2777
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发表时间：
2006-10-01
影响因子：
--
通讯作者：
Tuszynski, Mark H.
中科院分区：
文献类型：
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作者：
Stokols, Shula;Sakamoto, Jeff;Tuszynski, Mark H.
虽然几种策略可以刺激脊髓损伤部位内的轴突再生，但轴突的生长通常是无序和随机的。引导和维持通过损伤部位再生的轴突的天然组织的生物相容性支架在增强损伤后神经系统的恢复方面可能是重要的。在这里，我们报告了一种新的制造工艺模板琼脂糖神经引导支架组成的单轴通道的精确直径和壁厚延伸通过其全长。当在脊髓损伤的体内模型中进行测试时，支架表现出与宿主组织的良好整合，并通过其通道支持线性轴突生长。此外，当装载有经基因工程改造以分泌脑源性神经营养因子（BDNF）的骨髓基质细胞时，线性穿透轴突的数量显著增加。模板化过程可用于制造用于中枢和外周神经损伤的神经引导支架，或需要线性取向通道的精确阵列的任何材料应用。
While several strategies can stimulate axonal regeneration within a site of spinal cord injury, the growth of axons is generally disorganized and random. Biocompatible scaffolds that guide and maintain the native organization of axons regenerating through an injury site could be of importance in enhancing recovery of the nervous system after injury. Here we report a novel fabrication process for templated agarose nerve guidance scaffolds composed of uniaxial channels of precise diameter and wall thickness extending through their full length. When tested in an in vivo model of spinal cord injury, scaffolds exhibit excellent integration with host tissue and support linear axonal growth through their channels. Further, when loaded with bone marrow stromal cells genetically engineered to secrete brain-derived neurotrophic factor (BDNF), the number of linear penetrating axons is significantly enhanced. The templating process can be useful in fabricating nerve guidance scaffolds for both central and peripheral nerve injuries, or any materials application requiring a precise array of linearly oriented channels.