Increased expression of ADAM33 and ADAM8 with disease progression in asthma
Increased expression of ADAM33 and ADAM8 with disease progression in asthma
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DOI：
10.1016/j.jaci.2006.12.665
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发表时间：
2007-04-01
影响因子：
14.2
通讯作者：
Hamid, Qutayba
中科院分区：
文献类型：
--
作者：
Foley, Susan C.;Mogas, Andrea K.;Hamid, Qutayba
背景：ADAM33 是一种解整合素和金属蛋白酶 33 基因，已被确定为哮喘和支气管高反应性的危险因素，并被认为是气道重塑的基因。 ADAM8 受到过敏原和 T(H)2 细胞因子的强烈诱导；目的：为了评估这些基因在哮喘发病机制中的重要性，并研究表达是否与疾病严重程度或肺功能恶化有关，我们测量了哮喘受试者和对照受试者的支气管活检中这两个基因的 mRNA 和蛋白表达。方法：从轻度冷冻支气管内活检中提取 RNA。患有哮喘的中度和重度成人以及对照组。患有中度和重度哮喘的受试者正在服用皮质类固醇。使用特定引物通过实时 RT-PCR 测量两个基因的 mRNA 转录本。通过石蜡切片免疫组化检测蛋白表达。结果：与轻度哮喘相比，中度和重度哮喘中 ADAM33 mRNA 表达显着升高（P
Background: ADAM33, a disintegrin and metalloproteinase 33 gene, has been identified as a risk factor for asthma and bronchial hyperresponsiveness and has been postulated as a gene for airway remodeling. ADAM8 is strongly induced by allergens and T(H)2 cytokines; in the lung in experimental asthma.Objectives: To assess the importance of these genes in asthma pathogenesis and to investigate whether expression relates to disease severity or deterioration in lung function, we measured the mRNA and protein expression of both genes in bronchial biopsies of subjects with asthma and control subjects.Methods: RNA was extracted from frozen endobronchial biopsies of mild. moderate, and severe adults with asthma and controls. Subjects with moderate and severe asthma were taking corticosteroids. The mRNA transcript of both genes was measured by real time RT-PCR using specific primers. Protein expression was examined by immunohistochemistry on paraffin sections.Results: ADAM33 mRNA expression was significantly higher in both moderate and severe asthma compared with mild asthma (P