Induction of caspase-dependent apoptosis in cultured cells by the avian coronavirus infectious bronchitis virus
Induction of caspase-dependent apoptosis in cultured cells by the avian coronavirus infectious bronchitis virus
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DOI：
10.1128/jvi.75.14.6402-6409.2001
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发表时间：
2001-07-01
影响因子：
5.4
通讯作者：
Liu, DX
中科院分区：
文献类型：
--
作者：
Liu, C;Xu, HY;Liu, DX
禽冠状病毒感染性支气管炎病毒（IBV）是鸡感染性支气管炎的病因，一种急性，高度传染性的病毒呼吸道疾病。 Vero细胞中IBV的复制会引起广泛的细胞质效应（CPE），导致整个单层破坏和感染细胞的死亡。在这项研究中，我们研究了急性IBV感染和潜在机制期间的细胞死亡过程。结果表明，坏死和凋亡都可能导致裂解IBV感染中感染细胞的死亡。在IBV感染的VERO细胞中检测到caspase依赖性凋亡，其特征在于染色体凝结，DNA片段化，caspase-3激活和聚（ADP-核糖）聚合酶降解。在培养基中添加一般caspase抑制剂Z-VAD-FMK显示出抑制了凋亡的标志，并在感染后16小时将病毒释放到培养基上。然而，在Vero细胞中IBV的坏死过程和生产性复制都受到抑制凋亡的严重影响。筛选11 IBV编码的蛋白质表明，58 kDa成熟的切割产物可以诱导瞬时表达蛋白质的细胞中凋亡变化。这项研究为越来越多的动物病毒列表添加了一个例子，这些动物病毒在复制周期中诱导凋亡。
Avian coronavirus infectious bronchitis virus (IBV) is the causative agent of chicken infectious bronchitis, an acute, highly contagious viral respiratory disease. Replication of IBV in Vero cells causes extensive cytopathic effects (CPE), leading to destruction of the entire monolayer and the death of infected cells. In this study, we investigated the cell death processes during acute IBV infection and the underlying mechanisms. The results show that both necrosis and apoptosis may contribute to the death of infected cells in lytic IBV infection. Caspase-dependent apoptosis, as characterized by chromosomal condensation, DNA fragmentation, caspase-3 activation, and poly(ADP-ribose) polymerase degradation, was detected in IBV-infected Vero cells. Addition of the general caspase inhibitor z-VAD-FMK to the culture media showed inhibition of the hallmarks of apoptosis and increase of the release of virus to the culture media at 16 h postinfection. However, neither the necrotic process nor the productive replication of IBV in Vero cells was severely affected by the inhibition of apoptosis. Screening of 11 IBV-encoded proteins suggested that a 58-kDa mature cleavage product could induce apoptotic changes in cells transiently expressing the protein. This study adds one more example to the growing list of animal viruses that induce apoptosis during their replication cycles.