Deletion of luxS gene mediated by λRed gene recombination technology reduces biofilm formation and stress resistance of Lactobacillus fermentum
Deletion of luxS gene mediated by λRed gene recombination technology reduces biofilm formation and stress resistance of Lactobacillus fermentum
复制标题
DOI：
10.1016/j.fbio.2022.101892
复制
发表时间：
2022-07
期刊：
影响因子：
5.2
通讯作者：
Yue Zhang;Yue Gu;Yanxue Zheng;Yan Wang;Lili Nie;R. Qiao;Yinfeng He
中科院分区：
文献类型：
--
作者：
Yue Zhang;Yue Gu;Yanxue Zheng;Yan Wang;Lili Nie;R. Qiao;Yinfeng He
本研究探讨了uxS基因对发酵乳杆菌生物膜形成及其抗逆性的影响。UxS基因的缺失与生物膜发育的生理特性、菌株耐药性、细菌形态、生物膜形态、功能基团类型、生物膜的主要成分以及相关基因的表达有关。因此，利用λRed基因重组技术构建了aluxS基因缺陷型菌株，并比较了WT和Δ菌株的生物被膜产量和抗逆性。该基因的缺失降低了发酵乳杆菌生物膜的形成及其对酸、胆盐、高温和高渗环境的抵抗力。此外，本工作还发现该基因的缺失影响了生物膜中功能基团的类型，同时下调了FabI、cysE、argR和PurD基因的表达，从而降低了生物膜中脂肪酸、胞外多糖、蛋白质和Edna的水平。相关分析发现，生物膜中的蛋白质是影响生物膜形成的最重要因素。综上所述，发酵乳杆菌的uxS基因参与了生物膜形成和抗逆性的调节。这些发现为研究有益细菌生物被膜的形成机制提供了理论依据。
This study investigated the effects of theluxSgene on the biofilm formation ofLactobacillusfermentumand its stress resistance. Deletion of theluxSgene was related to the physiological characteristics of biofilm development, strain resistance, bacterial morphology, biofilm morphology, functional group types, the main components of the biofilm, and the expression of related genes. Therefore, aluxSgene-deficient strain was constructed using λRed gene recombination technology, and the biofilm production and stress resistance of the WT and ΔluxSstrains were compared. Deletion of the gene reduced the biofilm formation ofL.fermentumand its resistance to acid, bile salt, high temperatures, and a hypertonic environment. Further, this work found that its deletion affects the types of functional groups in biofilms, and at the same time downregulate the expressions of thefabI,cysE,argR, andpurDgenes, thus reducing the levels of fatty acids, exopolysaccharide, protein, and eDNA in biofilms. A correlation analysis found that protein in biofilms was the most important factor affecting biofilm formation. In conclusion, theluxSgene inL.fermentumis involved in the regulation of biofilm formation and stress resistance. These findings provide a theoretical basis for the study of the mechanism of biofilm formation in beneficial bacteria.