Nek1 shares structural and functional similarities with NIMA kinase
Nek1 shares structural and functional similarities with NIMA kinase
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DOI：
10.1016/j.bbamcr.2006.01.009
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发表时间：
2006-03-01
影响因子：
5.1
通讯作者：
Motro, Benny
中科院分区：
文献类型：
--
作者：
Feige, Erez;Shalom, Ohad;Motro, Benny
曲霉NIMA丝氨酸/苏氨酸激酶在控制进入有丝分裂中起关键作用。NIMA的一个主要功能是诱导染色质凝聚。我们在这里表明，创始人鼠NIMA相关激酶，Nek 1，是大于以前报道的，全长蛋白质保存NIMA的结构特征。尽管Nek 1具有两个经典的核定位信号（NLS），但内源性蛋白定位于细胞质。各种Nek 1构建体的异位过表达表明Nek 1的C-末端具有细胞质定位信号。Nek 1核结构的过表达导致异常染色质凝聚，DNA主要局限于细胞核的周边。晚期浓缩表型与核孔复合体分散有关。冷凝不伴随有丝分裂或凋亡标记物的上调。一个类似的表型已被描述NIMA过表达，加强了哺乳动物Nek 1激酶与NIMA具有功能相似性的概念。(c)2006 Elsevier B. V.保留所有权利。
The Aspergillus NIMA serine/threonine kinase plays a pivotal role in controlling entrance into mitosis. A major function attributed to NIMA is the induction of chromatin condensation. We show here that the founder murine NIMA-related kinase, Nek1, is larger than previously reported, and that the full-length protein conserves the structural hallmarks of NIMA. Even though Nek1 bears two classical nuclear localization signals (NLS), the endogenous protein localizes to the cytoplasm. Ectopic overexpression of various Nek1 constructs suggests that the C-terminus of Nek1 bears cytoplasmic localization signal(s). Overexpression of nuclear constructs of Nek1 resulted in abnormal chromatin condensation, with the DNA mainly confined to the periphery of the nucleus. Advanced condensation phenotype was associated with nuclear pore complex dispersal. The condensation was not accompanied by up-regulation of mitotic or apoptotic markers. A similar phenotype has been described following NIMA overexpression, strengthening the notion that the mammalian Nek1 kinase has functional similarity to NIMA. (c) 2006 Elsevier B.V. All rights reserved.