OsSYMRK Plays an Essential Role in AM Symbiosis in Rice (Oryza sativa)
OsSYMRK Plays an Essential Role in AM Symbiosis in Rice (Oryza sativa)
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OsSYMRK 在水稻 AM 共生中发挥重要作用 (Oryza sativa)
DOI：
10.1093/pcp/pcad006
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发表时间：
2023
影响因子：
4.9
通讯作者：
Kaku Hanae
中科院分区：
文献类型：
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作者：
Miyata Kana;Hosotani Moe;Akamatsu Akira;Takeda Naoya;Jiang Wendi;Sugiyama Taisei;Takaoka Ryou;Matsumoto Kotarou;Abe Satsuki;Shibuya Naoto;Kaku Hanae
丛枝菌根（AM）真菌与包括水稻在内的多种陆生植物建立互惠共生关系。然而，AM共生的启动机制尚未阐明，特别是在非豆科植物。我们以前证明，几丁质激发受体激酶1（OsCERK 1），一个溶素基序受体样激酶几丁质触发的免疫必不可少的，也起着关键作用，在水稻AM共生。然而，OsCERK 1调节免疫和共生之间切换的机制尚未完全阐明。共生受体样激酶（SYMRK）/不进行感染2（DMI 2）是一种富含亮氨酸重复序列的受体样激酶，与豆科植物的根瘤共生和AM共生有关。水稻中SYMRK的同源物OsSYMRK由于缺少malectin-like结构域（MLD）而具有比豆类中更短的形式。据报道，MLD有助于共生在莲花，然而，OsSYMRK的AM共生在水稻中的贡献还不清楚。系统发育分析表明，SYMRK/DMI 2的MLD是广泛保守的，即使在苔藓和蕨类植物，但缺乏鸭茅科，包括水稻。为了了解OsSYMRK的功能，我们利用基因组编辑技术制备了Ossymrk敲除突变体。AM定植在Ossymrk中大部分被消除，表型比Oscerk 1更严重。在Ossymrk中，Ca 2+对几丁质四聚体的刺激也减少了。相反，在Ossymrk中观察到对几丁质七聚体的与野生型相当的防御反应。双分子荧光互补研究表明OsSYMRK和OsCERK 1之间的相互作用表明OsSYMRK可能通过与OsCERK 1的相互作用在水稻从免疫到共生的转换中发挥重要作用。
Arbuscular mycorrhizal (AM) fungi establish mutualistic symbiosis with a wide range of terrestrial plants, including rice. However, the mechanisms underlying the initiation of AM symbiosis are yet to be elucidated, particularly in nonleguminous plants. We previously demonstrated that chitin elicitor receptor kinase 1 (OsCERK1), a lysin motif receptor–like kinase essential for chitin-triggered immunity, also plays a key role in AM symbiosis in rice. However, the mechanisms underlying the regulation of switching between immunity and symbiosis by OsCERK1 are yet to be fully elucidated. SYMBIOSIS RECEPTOR–LIKE KINASE (SYMRK)/DOES NOT MAKE INFECTIONS 2 (DMI2) is a leucine-rich repeat receptor–like kinase associated with both root nodule symbiosis and AM symbiosis in legumes. The homolog of SYMRK in rice, OsSYMRK, has a shorter form than that in legumes because OsSYMRK lacks a malectin-like domain (MLD). The MLD reportedly contributes to symbiosis inLotus japonicus; however, the contribution of OsSYMRK to AM symbiosis in rice remains unclear. Phylogenetic analyses indicated that the MLD of SYMRK/DMI2 is widely conserved even in mosses and ferns but absent in commelinids, including rice. To understand the function of OsSYMRK, we produced anOssymrkknockout mutant using genome editing technology. AM colonization was mostly abolished inOssymrkwith a more severe phenotype thanOscerk1. Ca2+spiking against chitin tetramer was also diminished inOssymrk. In contrast, comparable defense responses against chitin heptamer to the wild type were observed inOssymrk. Bimolecular fluorescence complementation studies demonstrating an interaction between OsSYMRK and OsCERK1 indicate that OsSYMRK may play an important role in switching from immunity to symbiosis through the interaction with OsCERK1 in rice.